Sakai Rika, Iguchi Hiroki, Maruyama Tatsuo
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University 1-1 Rokkodai, Nada-ku Kobe 657-8501 Japan
RSC Adv. 2019 Feb 18;9(8):4621-4625. doi: 10.1039/c8ra09421g. eCollection 2019 Jan 30.
Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent a cleavage reaction, which can be simply quantified with a conventional fluorometer.
在此，我们提出了一种使用可点击且可裂解的荧光化合物对固体表面和蛋白质上的叠氮基团进行定量的新方法。该可点击且可裂解的荧光化合物与材料表面的叠氮基团结合，然后通过裂解反应将荧光团释放到溶剂中，这可以用传统的荧光计进行简单定量。
