Institute for Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, DE-97074 Würzburg, Germany.
Institute for Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, DE-97074 Würzburg, Germany.
J Control Release. 2018 Mar 10;273:68-85. doi: 10.1016/j.jconrel.2018.01.018. Epub 2018 Jan 31.
Emerging strategies targeting site-specific protein modifications allow for unprecedented selectivity, fast kinetics and mild reaction conditions with high yield. These advances open exciting novel possibilities for the effective bioorthogonal decoration of biomaterials with therapeutic proteins. Site-specificity is particularly important to the therapeutics' end and translated by targeting specific functional groups or introducing new functional groups into the therapeutic at predefined positions. Biomimetic strategies are designed for modification of therapeutics emulating enzymatic strategies found in Nature. These strategies are suitable for a diverse range of applications - not only for protein-polymer conjugation, particle decoration and surface immobilization, but also for the decoration of complex biomaterials and the synthesis of bioresponsive drug delivery systems. This article reviews latest chemical and enzymatic strategies for the biorthogonal decoration of biomaterials with therapeutic proteins and inter-positioned linker structures. Finally, the numerous reports at the interface of biomaterials, linkers, and therapeutic protein decoration are integrated into practical advice for design considerations intended to support the selection of productive ligation strategies.
新兴的靶向特定部位蛋白质修饰的策略允许以前所未有的选择性、快速动力学和温和的反应条件实现高产率。这些进展为治疗性蛋白质对生物材料的有效生物正交修饰开辟了令人兴奋的新可能性。特异性在治疗学中尤为重要，通过靶向特定的功能基团或在预定位置引入新的功能基团来实现。仿生策略是为了模仿自然界中发现的酶策略而设计的治疗方法的修饰。这些策略适用于各种应用——不仅适用于蛋白质-聚合物偶联、颗粒修饰和表面固定化，还适用于复杂生物材料的修饰和生物响应性药物传递系统的合成。本文综述了最新的化学和酶策略，用于治疗性蛋白质与生物材料的生物正交修饰和中间连接子结构。最后，将生物材料、连接子和治疗性蛋白质修饰界面的众多报告整合到设计考虑因素的实际建议中，旨在支持有生产力的连接策略的选择。
