一种用于监测次氯酸的新型高特异性超灵敏荧光探针及其在活细胞中的应用。

A novel highly specific and ultrasensitive fluorescent probe for monitoring hypochlorous acid and its application in live cells.

作者信息

Li Xinyuan, Wu Liu, Zhao Ziyang, Liu Caiyun, Zhu Baocun

机构信息

Class 15, Grade 2 (64), Laiyang No. 1 High School Laiyang 265200 P. R. China.

School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization Jinan 250022 P. R. China

出版信息

RSC Adv. 2019 Feb 6;9(8):4659-4664. doi: 10.1039/c8ra09551e. eCollection 2019 Jan 30.

Based on the important role of hypochlorous acid (HOCl) in the immune system and numerous physiological processes, the detection of intracellular basal HOCl is of significant interest. In this work, we present a simple thiocarbamate-protected fluorescein fluorescent probe, TCFL, for imaging basal HOCl in live cells. Surprisingly, probe TCFL could determine HOCl quantitatively in a large concentration range with a detection limit of 0.65 nM. In addition, probe TCFL showed excellent specificity for HOCl in the presence of other higher concentration analytes (1 mM). Moreover, probe TCFL exhibited a rapid response (within 3 s) to HOCl and thus could provide a tool for real-time monitoring of HOCl. Importantly, probe TCFL with outstanding response features could be applied for monitoring basal HOCl in live cells.

基于次氯酸（HOCl）在免疫系统和众多生理过程中的重要作用，细胞内基础HOCl的检测备受关注。在这项工作中，我们展示了一种简单的硫代氨基甲酸盐保护的荧光素荧光探针TCFL，用于对活细胞中的基础HOCl进行成像。令人惊讶的是，探针TCFL能够在大浓度范围内对HOCl进行定量测定，检测限为0.65 nM。此外，在存在其他高浓度分析物（1 mM）的情况下，探针TCFL对HOCl表现出优异的特异性。而且，探针TCFL对HOCl表现出快速响应（在3秒内），因此可为HOCl的实时监测提供一种工具。重要的是，具有出色响应特性的探针TCFL可用于监测活细胞中的基础HOCl。