Wu Wei, Sun Ning, Zhu Simin, Li Xiaomei, Chen Jitao, Liu Chunzhao, Fang Zhiyuan, Yang Qingli
College of Food Science and Engineering, Qingdao Agricultural University No. 700 Changcheng Road Qingdao 266179 China
The Fifth Affiliated Hospital of Guangzhou Medical University No. 78, Hengzhigang Road Guangzhou 510095 Guangdong Province China
RSC Adv. 2019 Jan 21;9(5):2812-2815. doi: 10.1039/c8ra10589h. eCollection 2019 Jan 18.
In this biosensor system, metabolite residues were derived by using a previous B-CBA synthesis method to label a biotin moiety for enrichment by streptavidin coated magnetic beads. Antibodies specific for derivatives were conjugated with carboxyl-modified barcode DNAs which were used as templates for strand displacement amplification (SDA). The assay can detect trace levels of 7.20 ppt of SEM, 11.58 ppt of AHD, 7.24 ppt of AOZ and 2.31 ppt of AMOZ, respectively.
在该生物传感器系统中，通过使用先前的B-CBA合成方法衍生代谢物残留，以标记生物素部分，用于通过链霉亲和素包被的磁珠进行富集。针对衍生物的特异性抗体与羧基修饰的条形码DNA缀合，这些DNA用作链置换扩增（SDA）的模板。该测定法分别可以检测到痕量水平的7.20 ppt的SEM、11.58 ppt的AHD、7.24 ppt的AOZ和2.31 ppt的AMOZ。
