Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Chem Commun (Camb). 2013 Jul 14;49(55):6164-6. doi: 10.1039/c3cc42790k.
A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.
建立了一种使用新型生物素衍生物的直接 ELISA 方法，用于快速检测氨基脲（SEM）。无需繁琐的提取程序，该方法可重复性地检测到低至 0.07 μg L(-1) 的 SEM。与竞争性 ELISA 相比，该检测方法具有更好的回收率和准确性。
