Lu Xuewen, Liang Xiaoling, Dong Jianghong, Fang Zhiyuan, Zeng Lingwen
Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China.
Institute of Environmental and Food Safety, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei, 430000, China.
Anal Bioanal Chem. 2016 Sep;408(24):6703-9. doi: 10.1007/s00216-016-9787-2. Epub 2016 Jul 20.
The use of potential mutagenic nitrofuran antibiotic in food animal production has been banned world-wide. Common methods for nitrofuran detection involve complex extraction procedures. In the present study, magnetic beads functionalized with antibody against nitrofuran derivative were used as both the extraction and color developing media in lateral flow biosensor. Derivatization reagent carboxybenzaldehyde is firstly modified with ractopamine. After reaction with nitrofuran metabolites, the resultant molecule has two functional groups: the metabolite moiety and the ractopamine moiety. Metabolite moiety is captured by the antibody that is coated on magnetic beads. This duplex is then loaded onto biosensor and ractopamine moiety is further captured by the antibody immobilized on the test zone of nitrocellulose membrane. Without tedious organic reagent-based extraction procedure, this biosensor was capable of visually detecting four metabolites simultaneously with a detection limit of 0.1 μg/L. No cross-reactivity was observed in the presence of 50 μg/L interferential components. Graphical abstract Derivatization of nitrofuran metabolites (AHD, AOZ, SEM, or AMOZ) and LFA detection of the derivative products.
在食用动物生产中使用潜在致突变性的硝基呋喃抗生素已在全球范围内被禁止。常见的硝基呋喃检测方法涉及复杂的提取程序。在本研究中，用硝基呋喃衍生物抗体功能化的磁珠被用作侧流生物传感器中的提取和显色介质。衍生化试剂羧基苯甲醛首先用莱克多巴胺进行修饰。与硝基呋喃代谢物反应后，所得分子有两个官能团：代谢物部分和莱克多巴胺部分。代谢物部分被包被在磁珠上的抗体捕获。然后将这种双链体加载到生物传感器上，莱克多巴胺部分被固定在硝酸纤维素膜测试区的抗体进一步捕获。无需基于繁琐有机试剂的提取程序，这种生物传感器能够同时目视检测四种代谢物，检测限为0.1μg/L。在存在50μg/L干扰成分的情况下未观察到交叉反应。图形摘要：硝基呋喃代谢物（AHD、AOZ、SEM或AMOZ）的衍生化及衍生化产物的LFA检测。
