Miao Pandeng, Liu Zhongdong, Guo Jun, Yuan Ming, Zhong Ruibo, Wang Liping, Zhang Feng
Grain College, Henan University of Technology Zhengzhou 450001 P. R. China
State Key Laboratory of Respiratory Disease, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital, Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University Guangzhou 511436 P. R. China
RSC Adv. 2019 Jun 6;9(31):17698-17705. doi: 10.1039/c9ra02460c. eCollection 2019 Jun 4.
Nitrite is a common food additive, however, its reduction product, nitrosamine, is a strong carcinogen, and hence the ultra-sensitive detection of nitrite is an effective means to prevent related cancers. In this study, different sized gold nanoparticles (AuNPs) were modified with -aminothiophenol (ATP) and naphthylethylenediamine (NED). In the presence of nitrite, satellite-like AuNPs aggregates formed the diazotization coupling reaction and the color of the system was changed by the functionalized AuNPs aggregates. The carcinogenic nitrite content could be detected by colorimetry according to the change in the system color. The linear concentration range of sodium nitrite was 0-1.0 μg mL and the detection limit was determined to be 3.0 ng mL. Compared with the traditional method, this method has the advantages of high sensitivity, low detection limit, good selectivity and can significantly lower the naked-eye detection limit to 3.0 ng mL. In addition, this method is suitable for the determination of nitrite in various foods. We think this novel designed highly sensitive nitrate nanosensor holds great market potential.
亚硝酸盐是一种常见的食品添加剂，然而，其还原产物亚硝胺是一种强致癌物，因此亚硝酸盐的超灵敏检测是预防相关癌症的有效手段。在本研究中，不同尺寸的金纳米颗粒（AuNPs）用对氨基硫酚（ATP）和萘乙二胺（NED）进行了修饰。在亚硝酸盐存在下，通过重氮化偶联反应形成卫星状AuNPs聚集体，功能化的AuNPs聚集体改变了体系颜色。根据体系颜色变化，采用比色法可检测致癌性亚硝酸盐含量。亚硝酸钠的线性浓度范围为0 - 1.0 μg/mL，检测限为3.0 ng/mL。与传统方法相比，该方法具有灵敏度高、检测限低以及选择性好等优点，并且能将肉眼检测限显著降低至3.0 ng/mL。此外，该方法适用于各种食品中亚硝酸盐的测定。我们认为这种新设计的高灵敏度硝酸盐纳米传感器具有巨大的市场潜力。
