Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education of China, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
The First Clinical College, Chongqing Medical University, Chongqing 400016, China.
Biosensors (Basel). 2023 Sep 1;13(9):863. doi: 10.3390/bios13090863.
Capsaicinoids, mostly from chili peppers, are widely used in daily life. Capsaicinoids are considered to be markers for the identification of illegal cooking oil (ICO), which is a serious threat to public health. The identification of capsaicinoids can help reveal food-related fraud, thereby safeguarding consumers' health. Here, a novel and ultrasensitive method was established with a signal amplification strategy for the detection of capsaicinoids. AuNPs@FeO nanocomposites were functionalized with 4-aminothiophenol (4-atp). After diazotization, 4-atp on AuNPs@FeO reacted with capsaicinoids and formed capsaicinoids-azo-atp-AuNPs@FeO. Ultimately, capsaicinoids-azo-atp-AuNPs@FeO was dropped onto the surface of a screen-printed carbon electrode (SPCE) and detected via the differential pulse voltammetry (DPV) method. AuNPs@FeO nanocomposites increased the specific surface area of the electrode. Moreover, the diazotization-coupling reaction enriched the analytes on the electrode surface. Liquid-liquid extraction was used for sample pretreatment. Under a pH value of 9.0 and concentration of 0.20 mol/L for the supporting electrolyte, the linearity of capsaicinoids in ICO is from 0.10 to 10.00 ng/mL, and the limit of detection (S/N = 3) is 0.05 ng/mL. This method is ultra-sensitive, reliable, and cost-effective for the detection of capsaicinoids. Herein, this method provides a promising tool for the identification of ICO.
辣椒素类物质主要来自辣椒，在日常生活中被广泛应用。辣椒素类物质被认为是非法食用油（ICO）鉴别的标志物，这对公众健康构成了严重威胁。对辣椒素类物质的识别有助于揭示与食品相关的欺诈行为，从而保护消费者的健康。在这里，建立了一种新的、超灵敏的方法，该方法采用信号放大策略用于检测辣椒素类物质。AuNPs@FeO 纳米复合材料通过 4-巯基苯胺（4-atp）功能化。重氮化后，AuNPs@FeO 上的 4-atp 与辣椒素类物质反应，并形成辣椒素类物质偶氮-ATP-AuNPs@FeO。最终，将辣椒素类物质偶氮-ATP-AuNPs@FeO 滴加到丝网印刷碳电极（SPCE）的表面，并通过差分脉冲伏安法（DPV）进行检测。AuNPs@FeO 纳米复合材料增加了电极的比表面积。此外，重氮化偶联反应在电极表面富集了分析物。采用液-液萃取法进行样品预处理。在 pH 值为 9.0 和支持电解质浓度为 0.20 mol/L 的条件下，ICO 中辣椒素类物质的线性范围为 0.10-10.00ng/mL，检测限（S/N = 3）为 0.05ng/mL。该方法具有超高灵敏度、可靠性和成本效益，可用于检测辣椒素类物质。在此，该方法为 ICO 的鉴定提供了一种很有前途的工具。
