提取物对东莨菪碱诱导的大鼠行为障碍和脑氧化应激的保护作用。
Protective effects of extract against scopolamine-induced behavioral disturbances and brain oxidative stress in rats.
作者信息
Hosseini Mir-Jamal, Mahmoodi Niloofar, Eskandari Javad, Bijani Soroush, Yazdinezhad Ali Reza, Anoush Mahdieh
机构信息
Zanjan Applied Pharmacology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
出版信息
Heliyon. 2022 Apr 19;8(4):e09295. doi: 10.1016/j.heliyon.2022.e09295. eCollection 2022 Apr.
ETHNOPHARMACOLOGICAL RELEVANCE
Alzheimer's disease (AD) as the most common type of dementia, is affecting the life of many senior individuals around the world. Vinca Waldst. & Kit. (V. ) as a middle east originated plant demonstrated antioxidant and antitumor effects. This plant traditionally used to treat diabetes and hypertension, but its mechanism remains unclear.
AIM OF THE STUDY
In the present study, post-treatment effects of V. on learning and memory functions, antioxidant cellular defense and oxidative stress were investigated using the scopolamine rat model of AD.
MATERIALS AND METHODS
Wistar male rats (170-190 g) were administered Scopolamine, an anti-muscarinic drug, (2 mg/kg) for 10 days followed by V. extract (200, 300 and 400 mg/kg) and/or donepezil (DON; 1 mg/kg, which were administered before behavioral studies for 10 consecutive days. All the rats were then subjected to Morris water maze (MWM) task. Biochemical parameters of oxidative stress were quantified using the whole brain.
RESULTS
Our data showed significant decrease performance in target quadrant in water maze task following administration of scopolamine (SCOP). Also, V. and DON, did not induce any neurotoxicity and hepatotoxic effects at the highest utilized doses in healthy rats. Treatment with V. extract (200&400 mg/kg) and DON improved memory performance significantly in comparison with AD rats. In addition, V. extract in AD rats exhibited a decrease in malondialdehyde (MDA) and protein carbonyl (PCO) levels and an increase in total antioxidant capacity (FRAP) and glutathione (GSH) amounts in brain and liver.
CONCLUSION
It seems that cholinergic deficits and oxidative stress are consistently associated with Alzheimer's disease (AD). V. in case of indole alkaloids and flavonoids confirms the potentials of this herb in management of oxidative stress, resorting synaptic acetylcholine level and improving cellular antioxidant resources.
民族药理学相关性
阿尔茨海默病（AD）作为最常见的痴呆类型，正在影响着世界各地许多老年人的生活。长春花属植物Vinca Waldst. & Kit.作为一种原产于中东的植物，具有抗氧化和抗肿瘤作用。这种植物传统上用于治疗糖尿病和高血压，但其作用机制尚不清楚。
研究目的
在本研究中，使用东莨菪碱诱导的大鼠AD模型，研究长春花属植物对学习记忆功能、抗氧化细胞防御和氧化应激的治疗后效应。
材料与方法
将体重170 - 190 g的雄性Wistar大鼠给予抗毒蕈碱药物东莨菪碱（2 mg/kg），持续10天，随后给予长春花属植物提取物（200、300和400 mg/kg）和/或多奈哌齐（DON；1 mg/kg），连续10天在行为学研究前给药。然后所有大鼠进行莫里斯水迷宫（MWM）实验。使用全脑定量氧化应激的生化参数。
结果
我们的数据显示，给予东莨菪碱（SCOP）后，水迷宫任务中目标象限的表现显著下降。此外，长春花属植物提取物和多奈哌齐在健康大鼠中使用的最高剂量下未诱导任何神经毒性和肝毒性作用。与AD大鼠相比，长春花属植物提取物（200和400 mg/kg）和多奈哌齐治疗显著改善了记忆表现。此外，AD大鼠中的长春花属植物提取物使脑和肝中的丙二醛（MDA）和蛋白质羰基（PCO）水平降低，总抗氧化能力（FRAP）和谷胱甘肽（GSH）含量增加。
结论
胆碱能缺陷和氧化应激似乎与阿尔茨海默病（AD）始终相关。长春花属植物中的吲哚生物碱和黄酮类化合物证实了这种草药在管理氧化应激、恢复突触乙酰胆碱水平和改善细胞抗氧化资源方面的潜力。
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