Sun MingNa, Tong Zhou, Dong Xu, Chu Yue, Wang Mei, Gao TongChun, Duan JinSheng
Institute of Plant Protection and Agro-Product Safety, Anhui Academy of Agricultural Sciences Hefei 230031 China
Key Laboratory of Agro-Product Safety Risk Evaluation (Hefei), Ministry of Agriculture Hefei 230031 China.
RSC Adv. 2019 Mar 28;9(18):9887-9892. doi: 10.1039/c8ra10455g.
Penflufen is a highly efficient, broad-spectrum succinate dehydrogenase inhibitor. Owing to the increasing pesticide resistance in recent years, the use of a new fungicide, penflufen, has become increasingly widespread. However, residues that remain in the environment after the use of penflufen have an impact on human health. It is worth noting that penflufen is a chiral pesticide. The differences of residue behaviors between two enantiomers in living organisms need to be systematically studied. In this paper, reversed-phase liquid chromatography-mass spectrometry (LC-MS) was used to separate the enantiomers of penflufen, and the absolute configuration of the enantiomer was analyzed. The LC-MS/MS methods for the analysis of penflufen enantiomers on wheat plants, spinach, and Chinese cabbage were established. The results of the recovery experiments showed that the average recovery of the two enantiomers was 78.5-99.8% and RSD was 0.4-7.3%, suggesting that the accuracy and precision of the method meet the requirements of pesticide residue analysis. The results of stereoselective degradation of penflufen in the three matrices showed that there was little difference in the degradation of the two enantiomers in wheat and cabbage, while -(+)-penflufen was degraded preferentially in spinach. This study provides data supporting the scientific use and safety evaluation of penflufen.
戊唑醇是一种高效、广谱的琥珀酸脱氢酶抑制剂。由于近年来农药抗性不断增加，新型杀菌剂戊唑醇的使用越来越广泛。然而，戊唑醇使用后残留在环境中的物质会对人体健康产生影响。值得注意的是，戊唑醇是一种手性农药。两种对映体在生物体内的残留行为差异需要进行系统研究。本文采用反相液相色谱-质谱联用（LC-MS）法分离戊唑醇对映体，并分析了对映体的绝对构型。建立了在小麦植株、菠菜和大白菜上分析戊唑醇对映体的LC-MS/MS方法。回收率实验结果表明，两种对映体的平均回收率为78.5-99.8%，相对标准偏差为0.4-7.3%，表明该方法的准确性和精密度符合农药残留分析要求。戊唑醇在三种基质中的立体选择性降解结果表明，两种对映体在小麦和白菜中的降解差异不大，而（+）-戊唑醇在菠菜中优先降解。本研究为戊唑醇的科学使用和安全性评价提供了数据支持。
