Li Jian, Bai Ding-Ping, Zhang Xi-Feng
Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University Fuzhou 350002 China.
College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University Wuhan 430023 China
RSC Adv. 2019 Jul 10;9(37):21513-21517. doi: 10.1039/c8ra09495k. eCollection 2019 Jul 5.
Due to the lack of a var. reference genome, we assembled a reference transcriptome from C. A. Mey transcriptome sequencing data, and 203 283 unigenes were obtained. In this study, with the assistance from the Trinity, Bowtie2 and SAMtools softwares, 218 465 single nucleotide polymorphisms (SNPs) were identified by mapping the Illumina sequences to the reference transcriptome. The SNP forms included 126 262 transformations and 92 203 transversions. A large number of SNP loci were associated with triterpenoid saponin synthesis: 54 SNPs were associated with cytochrome P450, one with glycosyl transferase and 94 with the biosynthesis of the triterpenoid saponin backbone.
由于缺乏变种参考基因组，我们从C. A. Mey转录组测序数据中组装了一个参考转录组，共获得203283个单基因。在本研究中，借助Trinity、Bowtie2和SAMtools软件，通过将Illumina序列映射到参考转录组，鉴定出218465个单核苷酸多态性（SNP）。SNP形式包括126262个转换和92203个颠换。大量SNP位点与三萜皂苷合成相关：54个SNP与细胞色素P450相关，1个与糖基转移酶相关，94个与三萜皂苷骨架的生物合成相关。
