An Chao, Ma Saijian, Shi Xinwei, Liu Chen, Ding Hao, Xue Wenjiao
Shaanxi Institute of Microbiology, Xi'an, China.
Engineering Center of QinLing Mountains Natural Products, Shaanxi Academy of Sciences, Xi'an, China.
Front Pharmacol. 2022 Apr 4;13:762862. doi: 10.3389/fphar.2022.762862. eCollection 2022.
To obtain novel fungi with potent β-glucosidase for minor ginsenoside production, var. , which is a traditional medicinal plant containing various ginsenosides, was first employed to isolate endophytic fungi in this study. A total of 93 representative morphotype strains were isolated and identified according to ITS rDNA sequence analyses, and they were grouped into three phyla (Ascomycota, Basidiomycota, and Mucoromycota), five classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Agaricomycetes, and Mucoromycetes), and 24 genera. (RA, 19.35%) was the most abundant genus, followed by (RA, 11.83%) and m (RA, 9.70%). The species richness index (S, 34) and the Shannon-Wiener index (', 3.004) indicated that harbored abundant fungal resources. A total of 26 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. In total, 11 strains showed strong β-glucosidase activities and also presented with the ability of ginsenoside biotransformation with varied glycoside-hydrolyzing pathways. Excitingly, three genera, namely, , , and , and all 11 taxa were first found to have the ability to transform ginsenosides in our study. The results indicated that could be a new fungi resource with potential novel natural compounds with antimicrobial activity and potent β-glucosidase for varied minor ginsenoside production.
为了获得具有强效β - 葡萄糖苷酶以生产稀有皂苷的新型真菌，本研究首先选用了含有多种皂苷的传统药用植物[植物名称未给出]来分离内生真菌。根据ITS rDNA序列分析，共分离并鉴定出93株具有代表性形态型的菌株，它们被分为三个门（子囊菌门、担子菌门和毛霉门）、五个纲（座囊菌纲、粪壳菌纲、散囊菌纲、伞菌纲和毛霉纲）和24个属。[属名未给出]（相对丰度，19.35%）是最丰富的属，其次是[属名未给出]（相对丰度，11.83%）和[属名未给出]（相对丰度，9.70%）。物种丰富度指数（S，34）和香农 - 维纳指数（H'，3.004）表明[植物名称未给出]含有丰富的真菌资源。共有26种内生真菌的乙酸乙酯提取物对至少一种病原菌或真菌表现出抑制活性。总共有11株菌株表现出较强的β - 葡萄糖苷酶活性，并且还具有通过不同糖苷水解途径进行人参皂苷生物转化的能力。令人兴奋的是，在我们的研究中首次发现三个属，即[属名未给出]、[属名未给出]和[属名未给出]，以及所有11个分类单元都具有转化人参皂苷的能力。结果表明，[植物名称未给出]可能是一种新的真菌资源，具有潜在的新型天然化合物，这些化合物具有抗菌活性以及用于生产多种稀有皂苷的强效β - 葡萄糖苷酶。
