Ramarao Kivaandra Dayaa Rao, Somasundram Chandran, Razali Zuliana, Kunasekaran Wijenthiran, Li Jin Tan
Institute of Biological Sciences, Faculty of Science, and Centre for Research in Biotechnology for Agriculture (CEBAR) Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Cytonex Sdn. Bhd, Menara Uoa Bangsar, A-16-17, No. 5, Jalan Bangsar Utama 1, Bangsar, 59000 Kuala Lumpur, Malaysia.
Saudi J Biol Sci. 2022 Jun;29(6):103290. doi: 10.1016/j.sjbs.2022.103290. Epub 2022 Apr 21.
The recent COVID-19 pandemic resulted in major postharvest losses because most fresh produce could not be sold. Drying is an important thermal-based food preservation method which could have prolonged the shelf-life of these produce, but most drying technologies are costly, and cannot be afforded by small-time farmers. From this context, we were interested in evaluating the drying of leaves (MOL) using a low-cost self-built prototype convective-air dryer (CAD), alongside conventional drying methods for its antioxidant properties, microbial load and phytoconstituents. Results showed total polyphenol content was the highest (p < 0.05) in our CAD samples, and it retained among the highest total flavonoid content, total antioxidant capacity, total alkaloid content and DPPH radical scavenging activity. Furthermore, methanolic CAD extract presented lower coliform and yeast and mold count than the aqueous CAD extract. We also briefly explored MOL as a sanitizer where the microbial load of the methanolic extract was comparable (p > 0.05) with several commercial non-alcoholic sanitizers, indicating its commercialization potential as a bio-friendly sanitizer. Finally, using GC-MS, we are the first to report (best of our knowledge) on the presence of caprolactam, an important bio-medical field compound, in the CAD sample's aqueous extract.
近期的新冠疫情导致了大量的收获后损失,因为大多数新鲜农产品无法售出。干燥是一种重要的基于热的食品保存方法,本可延长这些农产品的保质期,但大多数干燥技术成本高昂,小农户负担不起。在此背景下,我们有兴趣使用低成本自制的对流空气干燥机(CAD)评估树叶(MOL)的干燥效果,并将其与传统干燥方法在抗氧化特性、微生物负荷和植物成分方面进行比较。结果显示,我们的CAD样品中总多酚含量最高(p < 0.05),并且在总黄酮含量、总抗氧化能力、总生物碱含量和DPPH自由基清除活性方面也保持在最高水平。此外,CAD甲醇提取物中的大肠菌群、酵母和霉菌数量低于水提取物。我们还简要探讨了MOL作为消毒剂的潜力,其甲醇提取物的微生物负荷与几种商业非酒精消毒剂相当(p > 0.05),这表明其作为生物友好型消毒剂的商业化潜力。最后,通过气相色谱 - 质谱联用(GC - MS),据我们所知,我们首次报告了CAD样品水提取物中存在己内酰胺,这是一种重要的生物医学领域化合物。