Nwidu Lucky Legbosi, Elmorsy Ekramy, Aprioku Jonah Sydney, Siminialayi Iyeopu, Carter Wayne Grant
Department of Experimental Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Port Harcourt PMB 5323, Rivers State, Nigeria.
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
Medicines (Basel). 2018 Jul 5;5(3):71. doi: 10.3390/medicines5030071.
This study evaluated extracts from two locations in Niger Delta for in vitro anti-cholinesterase and antioxidant activities. Methanolic, aqueous and ethanolic extracts of were evaluated for inhibition of acetylcholinesterase (AChE) activity, antioxidant properties, and total phenolic and flavonoid contents using standard procedures. extracts possessed significant and concentration dependent AChE inhibitory activity for methanolic, aqueous, and ethanolic extracts. For the most potent extracts, the percentage AChE inhibition/IC (µg/mL) values were root methanolic extracts (MORME): ~80%/0.00845; root ethanolic extract 1 (MOREE1): ~90%/0.0563; root ethanolic extract 2 (MOREE2): ~70%/0.00175; and bark ethanolic extract (MOBEE): ~70%/0.0173. The descending order of AChE inhibitory potency of plant parts were: root > bark > leaf > flowers > seed. All methanolic extracts at a concentration of 1000 µg/mL displayed significant ( < 0.05⁻0.001) DPPH radical scavenging activity, with values of ~20⁻50% of that of ascorbic acid. The total phenolic content and total flavonoid content (TPC/TFC) of MORME, bark methanolic extract (MOBME), MOREE1, MOREE2 and Moringa leaf ethanolic leaf extract (MLEE) were (287/254), (212/113), (223/185), (203/343) and (201/102) mg gallic acid equivalents/g and quercetin equivalents/g, respectively. There was an inverse correlation between plant extract AChE inhibition and total phenolic ( < 0.0001) and total flavonoid contents ( < 0.0012). In summary, this study revealed 5 of 19 extracts of that have potent in vitro anti-cholinesterase and antioxidant activities.
本研究评估了来自尼日尔三角洲两个地点的提取物的体外抗胆碱酯酶和抗氧化活性。使用标准程序对提取物的甲醇、水和乙醇提取物进行了乙酰胆碱酯酶（AChE）活性抑制、抗氧化性能以及总酚和黄酮含量的评估。提取物的甲醇、水和乙醇提取物具有显著的、浓度依赖性的AChE抑制活性。对于最有效的提取物，AChE抑制率/IC（μg/mL）值分别为：根甲醇提取物（MORME）：约80%/0.00845；根乙醇提取物1（MOREE1）：约90%/0.0563；根乙醇提取物2（MOREE2）：约70%/0.00175；树皮乙醇提取物（MOBEE）：约70%/0.0173。植物各部位AChE抑制效力的降序排列为：根＞树皮＞叶＞花＞种子。所有浓度为1000μg/mL的甲醇提取物均表现出显著（P＜0.05⁻0.001）的DPPH自由基清除活性，其值约为抗坏血酸的20⁻50%。MORME、树皮甲醇提取物（MOBME）、MOREE1、MOREE2和辣木叶乙醇提取物（MLEE）的总酚含量和总黄酮含量（TPC/TFC）分别为（287/254）、（212/113）、（223/185）、（203/343）和（201/102）mg没食子酸当量/g和槲皮素当量/g。植物提取物的AChE抑制与总酚（P＜0.0001）和总黄酮含量（P＜0.0012）之间存在负相关。总之，本研究揭示了19种辣木提取物中的5种具有强大的体外抗胆碱酯酶和抗氧化活性。
