Cai Xuekang, Wang Dan, Gao Yasi, Yi Long, Yang Xing, Xi Zhen
Department of Nuclear Medicine, Peking University First Hospital Beijing 100034 China
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology 15 Beisanhuan East Road, Chaoyang District Beijing 100029 China.
RSC Adv. 2018 Dec 19;9(1):23-26. doi: 10.1039/c8ra09303b.
We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M s, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.
我们通过四氟芳香叠氮化物开发了一种快速的应变促进叠氮化物-炔烃环加成反应（SPAAC），其动力学常数为3.60 M s，这是迄今为止报道的最快的SPAAC连接反应之一。我们成功地将该反应用于蛋白质的高效共价标记以及活细胞中线粒体的生物成像。该反应可能是化学生物学和生物材料领域普遍有用的工具。
