Guo Na, Bai Yuxin, Huang Xin, Liu Xiaokang, Cai Guangzhi, Liu Shuying, Guo Yunlong, Gong Jiyu
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China.
Int J Anal Chem. 2022 Apr 26;2022:6721937. doi: 10.1155/2022/6721937. eCollection 2022.
A method with ultrahigh performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was applied for the quality evaluation of different processing and drying of American ginseng, including natural drying (ND), steam drying (SD), and vacuum freeze-drying (VFD). A total of 51 saponins were successfully identified in three processed products. Three processed American ginseng products were well-differentiated in orthogonal partial least-squares discriminant analysis (OPLS-DA). The S-plot also identified the marker compounds in each product, while the major ginsenosides of ND (malonyl (M)-Rd, M-Rb, Rg), SD (20 (S)-Rg, 20 (S)-Rg), and VFD (M-Rd, M-Rb) were found. The results indicate that the method by vacuum freeze-drying can retain the content of rare ginsenosides and malonyl-ginsenosides. The marker compounds selected will benefit the holistic evaluation of related American ginseng products.
采用超高效液相色谱四极杆-轨道阱串联质谱法（UHPLC-Q-Orbitrap-MS/MS）对西洋参不同加工和干燥方式（包括自然干燥（ND）、蒸汽干燥（SD）和真空冷冻干燥（VFD））进行质量评价。在三种加工产品中成功鉴定出51种皂苷。在正交偏最小二乘法判别分析（OPLS-DA）中，三种加工的西洋参产品得到了很好的区分。S-图还确定了每种产品中的标志性化合物，同时发现了自然干燥产品（丙二酰（M）-Rd、M-Rb、Rg）、蒸汽干燥产品（20（S）-Rg、20（S）-Rg）和真空冷冻干燥产品（M-Rd、M-Rb）中的主要人参皂苷。结果表明，真空冷冻干燥方法可以保留稀有人参皂苷和丙二酰人参皂苷的含量。所选择的标志性化合物将有助于对相关西洋参产品进行整体评价。
