State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing, 210096, P. R. China.
Adv Healthc Mater. 2022 Jul;11(14):e2200453. doi: 10.1002/adhm.202200453. Epub 2022 May 12.
Direct, noninvasive, and real-time imaging of Staphylococcus aureus (S. aureus) infection is of great value for quick diagnosis of related disease in clinic, but remains challenging. Herein, employing a rationally designed near-infrared fluorescence probe Cys(StB u)-EDA-Thioketal-Lys(Cy5.5)-CBT (TK-CBT) and a CBT-Cys click reaction, the fluorescence-quenched nanoparticles TK-CBT-NPs are facilely prepared. Upon oxidation by the abundant reactive oxygen species in S. aureus-infected macrophages, TK-CBT-NPs are fractured, turning the fluorescence "on" for imaging infections in vitro and in vivo. Specifically, TK-CBT-NPs show a 6.1-fold fluorescence imaging signal enhancement of the macrophages that are infected by S. aureus for 20 h in vitro. At 4 h postinjection, TK-CBT-NPs show a 2.8-fold fluorescence imaging signal enhancement of the sites in mice that are infected by S. aureus for 24 h. It is anticipated that TK-CBT-NPs could be applied for diagnosis of S. aureus infections in clinic in the near future.
直接、非侵入性和实时的金黄色葡萄球菌（S. aureus）感染成像对于临床中相关疾病的快速诊断具有重要价值，但仍然具有挑战性。在此，通过合理设计的近红外荧光探针 Cys(StB u)-EDA-Thioketal-Lys(Cy5.5)-CBT（TK-CBT）和 CBT-Cys 点击反应，简便地制备了荧光猝灭纳米颗粒 TK-CBT-NPs。在金黄色葡萄球菌感染的巨噬细胞中丰富的活性氧的氧化作用下，TK-CBT-NPs 发生断裂，从而使荧光“开启”，实现体外和体内感染的成像。具体而言，TK-CBT-NPs 在体外金黄色葡萄球菌感染 20 h 的巨噬细胞中表现出 6.1 倍的荧光成像信号增强。在注射后 4 h，TK-CBT-NPs 在金黄色葡萄球菌感染 24 h 的小鼠部位表现出 2.8 倍的荧光成像信号增强。预计 TK-CBT-NPs 可在不久的将来应用于临床金黄色葡萄球菌感染的诊断。
