Department of Chemical and Biomolecular Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, Maryland 21218, United States.
Department of Chemical Engineering and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
Nano Lett. 2022 May 25;22(10):4182-4191. doi: 10.1021/acs.nanolett.2c00967. Epub 2022 May 6.
Spraying serves as an attractive, minimally invasive means of administering hydrogels for localized delivery, particularly due to high-throughput deposition of therapeutic depots over an entire target site of uneven surfaces. However, it remains a great challenge to design systems capable of rapid gelation after shear-thinning during spraying and adhering to coated tissues in wet, physiological environments. We report here on the use of a collagen-binding peptide to enable a supramolecular design of a biocompatible, bioadhesive, and sprayable hydrogel for sustained release of therapeutics. After spraying, the designed peptide amphiphile-based supramolecular filaments exhibit fast, physical cross-linking under physiological conditions. Our studies suggest that the hydrogelator strongly adheres to the wet surfaces of multiple organs, and the extent of binding to collagen influences release kinetics from the gel. We envision that the sprayable organ-adhesive hydrogel can serve to enhance the efficacy of incorporated therapeutics for many biomedical applications.
喷雾是一种有吸引力的、微创的水凝胶给药方法，特别适用于在整个不均匀表面的目标部位进行高通量沉积治疗库。然而，设计能够在喷雾过程中剪切变稀后迅速胶凝并在湿生理环境中粘附在涂层组织上的系统仍然是一个巨大的挑战。我们在这里报告了使用胶原结合肽来实现一种生物相容的、生物粘附的、可喷雾的水凝胶的超分子设计，用于治疗药物的持续释放。喷雾后，设计的基于肽两亲物的超分子纤维在生理条件下表现出快速的物理交联。我们的研究表明，水凝胶剂强烈地粘附在多个器官的湿表面上，并且与胶原的结合程度影响从凝胶中的释放动力学。我们设想可喷雾的器官粘附水凝胶可以用于增强许多生物医学应用中包含的治疗药物的疗效。
