Yamada M, Okigaki T, Awai M
Cell Struct Funct. 1987 Feb;12(1):53-62. doi: 10.1247/csf.12.53.
We previously reported that proteins from serum-free conditioned medium (PCM) of rat embryo fibroblasts promote the adhesion of nonmalignant rat liver epithelial cells (RL34) seeded on a collagen substratum (Yamada, M. and Okigaki, T., Cell Biol. Int. Rep. 7, 1115(1983)). We now have compared the adhesion and growth of RL34 cells seeded on an extracellular matrix (RLECM), a product of RL34 cells, or on a collagen (type I) substratum with or without PCM in the medium. The adhesion rate for RL34 cells on RLECM was higher than on collagen, and once RL34 cells adhered to the collagen substratum they showed no growth, whether or not PCM was present. Cells that adhered to RLECM grew, and the growth rate was markedly higher in medium containing PCM than in medium without it. Immunological, enzymatic and chemical analyses revealed that the adhesion- and growth-promoting activities of RLECM involved trypsin-sensitive proteins other than collagens, fibronectin (FN) or laminin (LN), and that heparan sulfate proteoglycan may be a major component of RLECM.
我们之前报道过，大鼠胚胎成纤维细胞无血清条件培养基（PCM）中的蛋白质可促进接种在胶原基质上的非恶性大鼠肝上皮细胞（RL34）的黏附（山田，M.和冲垣，T.，《细胞生物学国际报告》7，1115(1983)）。我们现在比较了接种在细胞外基质（RLECM，一种RL34细胞的产物）、I型胶原基质上的RL34细胞在有或无PCM的培养基中的黏附与生长情况。RL34细胞在RLECM上的黏附率高于在胶原上的黏附率，并且一旦RL34细胞黏附到胶原基质上，无论有无PCM，它们都不生长。黏附到RLECM上的细胞会生长，并且在含有PCM的培养基中的生长速率明显高于不含PCM的培养基。免疫学、酶学和化学分析表明，RLECM的黏附促进和生长促进活性涉及除胶原、纤连蛋白（FN）或层粘连蛋白（LN）之外的对胰蛋白酶敏感的蛋白质，并且硫酸乙酰肝素蛋白聚糖可能是RLECM的主要成分。
