肺炎球菌转化中供体脱氧核糖核酸长度及标记效应
Donor deoxyribonucleic acid length and marker effect in pneumococcal transformation.
作者信息
Lefevre J C, Claverys J P, Sicard A M
出版信息
J Bacteriol. 1979 Apr;138(1):80-6. doi: 10.1128/jb.138.1.80-86.1979.
Abstract
The efficiency of transformation of point mutations depends upon base pair mismatches during the recombination process. For low-efficiency markers, the genetic information carried on the donor deoxyribonucleic acid is preferentially lost. To understand this elimination process, we investigated the effect of the size of donor deoxyribonucleic acid on the relative efficiency of low-efficiency point mutations. The deoxyribonucleic acid was shortened either by mechanical shearing or by restriction enzyme treatments. The results indicate that transformation by low-efficiency markers was not affected by shortening the distance between them and the end of the molecule any more than was transformation by the other markers. Moreover, no lethal event could be detected for either cell or chromosomal marker survival. These data do not exclude the double-strand-break hypothesis that was proposed to explain the loss of genetic information for low-efficiency markers, but they offer no support for it.
摘要
点突变的转化效率取决于重组过程中的碱基对错配。对于低效标记，供体脱氧核糖核酸携带的遗传信息优先丢失。为了解这一消除过程，我们研究了供体脱氧核糖核酸大小对低效点突变相对效率的影响。通过机械剪切或限制性内切酶处理使脱氧核糖核酸缩短。结果表明，与其他标记的转化相比，低效标记的转化不会因缩短它们与分子末端之间的距离而受到更多影响。此外，无论是细胞还是染色体标记存活，均未检测到致死事件。这些数据并不排除为解释低效标记的遗传信息丢失而提出的双链断裂假说，但也未提供支持。
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