Czuprynski C J, Henson P M, Campbell P A
J Leukoc Biol. 1984 Feb;35(2):193-208. doi: 10.1002/jlb.35.2.193.
Acquired resistance to the facultative intracellular bacterium Listeria monocytogenes is thought to require immunologically activated macrophages. Using peritoneal exudate cells from nonimmunized mice in a suspension bactericidal assay, however, we found that peritoneal neutrophils obtained early during the inflammatory process (4 hr after elicitation) and macrophages obtained later during inflammation (maximal listericidal activity at 48 hr after elicitation) were able to kill Listeria in vitro. The kinetics of expression of bactericidal activity by inflammatory neutrophils and macrophages against both L monocytogenes and E coli were similar. Although intraperitoneal immunization or intravenous hyperimmunization markedly enhanced resistance of mice to Listeria in vivo, immunization did not increase the ability of inflammatory peritoneal phagocytes to kill Listeria in vitro. However, in response to intraperitoneal injection of proteose-peptone or dead Listeria, immunized mice mobilized more neutrophils and monocytes into the inflamed peritoneum. These data suggest that, rather than systemic activation of mononuclear phagocyte bactericidal activity, increased mobilization of neutrophils and mononuclear phagocytes into sites of infection may be of prime importance in resistance to listeriosis.
对兼性胞内细菌单核细胞增生李斯特菌产生获得性抗性被认为需要免疫激活的巨噬细胞。然而，在悬浮杀菌试验中使用未免疫小鼠的腹腔渗出细胞，我们发现炎症过程早期（激发后4小时）获得的腹腔中性粒细胞以及炎症后期（激发后48小时达到最大杀菌活性）获得的巨噬细胞能够在体外杀死李斯特菌。炎症中性粒细胞和巨噬细胞对单核细胞增生李斯特菌和大肠杆菌的杀菌活性表达动力学相似。尽管腹腔内免疫或静脉内超免疫显著增强了小鼠在体内对李斯特菌的抗性，但免疫并未增加炎症性腹腔吞噬细胞在体外杀死李斯特菌的能力。然而，响应于腹腔内注射蛋白胨或死的李斯特菌，免疫小鼠动员了更多的中性粒细胞和单核细胞进入发炎的腹膜。这些数据表明，对于抗李斯特菌病而言，增加中性粒细胞和单核细胞向感染部位的动员可能比单核吞噬细胞杀菌活性的全身激活更为重要。
