Watabe S, Sendo F, Kimura S, Arai S
J Natl Cancer Inst. 1984 Jun;72(6):1365-70.
Injection ip of a streptococcal preparation, OK-432, into WKA and DONRYU rats induced in vitro cytotoxicity of polymorphonuclear leukocytes (PMN) against tumor cells in terms of their cytostasis and cytolysis. PMN were obtained from peritoneal exudates of WKA and DONRYU rats. Cytotoxicity of activated PMN was immunologically nonspecific, although a certain target selectivity was observed in PMN cytotoxicity. The titer of cytotoxicity of PMN was dependent on the dose of injected OK-432 and on the number of PMN in the cytotoxicity assay. Activated PMN appeared very early (6 hr) after OK-432 injection, and the cytotoxic titer of PMN decreased from 24 hours and disappeared at 96 hours after injection of the reagent. In vitro culture of PMN with OK-432 also evoked cytotoxicity of PMN.
向WKA和DONRYU大鼠腹腔注射链球菌制剂OK-432，可诱导多形核白细胞（PMN）对肿瘤细胞产生体外细胞毒性，表现为细胞生长抑制和细胞溶解。PMN取自WKA和DONRYU大鼠的腹腔渗出液。活化的PMN的细胞毒性在免疫上是非特异性的，尽管在PMN细胞毒性中观察到一定的靶标选择性。PMN的细胞毒性效价取决于注射的OK-432剂量以及细胞毒性测定中PMN的数量。注射OK-432后，活化的PMN很早就出现了（6小时），并且PMN的细胞毒性效价在注射试剂后24小时开始下降，并在96小时时消失。用OK-432对PMN进行体外培养也可诱发PMN的细胞毒性。
