Key Laboratory for Organic Electronics and Information Displays (KLOEID), Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing, 210023, P. R. China.
School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.
Small. 2022 Jun;18(22):e2200824. doi: 10.1002/smll.202200824. Epub 2022 May 6.
Constructing high-order DNA nano-architectures in large sizes is of critical significance for the application of DNA nanotechnology. Robust and flexible design strategies together with easy protocols to construct high-order large-size DNA nano-architectures remain highly desirable. In this work, the authors report a simple and versatile one-pot strategy to fabricate DNA architectures with the assistance of spherical gold nanoparticles modified with thiolated oligonucleotide strands (SH-DNA-AuNPs), which serve as "power strips" to connect various DNA nanostructures carrying complementary ssDNA strands as "plugs". By modulating the plug numbers and positions on each DNA nanostructure and the ratios between DNA nanostructures and AuNPs, the desired architectures are formed via the stochastic co-assembly of different modules. This SH-DNA-AuNP-mediated plug-in assembly (SAMPA) strategy offers new opportunities to drive macroscopic self-assembly to meet the demand of the fabrication of well-defined nanomaterials and nanodevices.
构建大规模的高阶 DNA 纳米结构对于 DNA 纳米技术的应用具有至关重要的意义。稳健且灵活的设计策略以及易于构建高阶大规模 DNA 纳米结构的方案仍然是非常需要的。在这项工作中，作者报道了一种简单而通用的一锅法策略，该策略借助于巯基化寡核苷酸链修饰的球形金纳米颗粒（SH-DNA-AuNPs）来制造 DNA 结构，这些颗粒作为“电源带”将带有互补 ssDNA 链的各种 DNA 纳米结构作为“插头”连接起来。通过调节每个 DNA 纳米结构上的插头数量和位置以及 DNA 纳米结构与 AuNPs 之间的比例，可以通过不同模块的随机共组装来形成所需的结构。这种 SH-DNA-AuNP 介导的插件组装（SAMPA）策略为驱动宏观自组装提供了新的机会，以满足制造具有明确定义的纳米材料和纳米器件的需求。
