Department of Immunology, Cellular and Molecular Research Center, Basic Health Sciences Institute, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2 Clinical Research Development Unit of Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
Iran J Allergy Asthma Immunol. 2022 Feb 6;21(1):20-26. doi: 10.18502/ijaai.v21i1.8609.
Interleukin (IL)-35 and IL-37 are two anti-inflammatory cytokines. IL-35 inhibits the development of T-effector cells such as Th1, and Th17; while increasing regulatory T cells (Tregs). IL-37 causes the suppression of inflammatory cytokines. Regarding the positive impact of Helicobacter pylori (H. pylori) infection on inflammation and considering the anti-inflammatory effects of IL-35 and IL-37, this study aimed to evaluate the expression of these two cytokines in H. pylori-infected patients with gastrointestinal problems. The case group consisted of H. pylori-infected individuals with gastric ulcer and/or gastritis (n=50) and the control group consisted of cases with gastric ulcer and/or gastritis non-H. pylori-infected (n=50). Sampling and classification of patients were based on pathology findings. A real-time polymerase chain reaction was performed for evaluating the IL-35 and IL-37 expression levels. pylori-infected gastritis patients showed lower expression of IL-35 and IL-37 than the non-infected group. There was a significant difference between the expression levels of IL-35 and IL-37 in patients with gastric ulcers and/or gastritis who were infected and non-infected by H. pylori. There were no significant differences in the expression level of IL-35 and IL-37 in H. pylori-infected patients with gastric ulcer or gastritis. Interleukins 37 and 35 were less expressed in patients with H. pylori-infection. In differentiation between patients with gastrointestinal symptoms who have H. pylori infection or with similar symptoms who do not have H. pylori-infection, mentioned interleukins can be used as diagnostic markers.
白细胞介素 (IL)-35 和 IL-37 是两种抗炎细胞因子。IL-35 抑制 Th1 和 Th17 等 T 效应细胞的发育；同时增加调节性 T 细胞 (Tregs)。IL-37 导致炎症细胞因子的抑制。鉴于幽门螺杆菌 (H. pylori) 感染对炎症的积极影响，并且考虑到 IL-35 和 IL-37 的抗炎作用，本研究旨在评估这两种细胞因子在患有胃肠道问题的 H. pylori 感染患者中的表达。病例组包括患有胃溃疡和/或胃炎的 H. pylori 感染患者（n=50），对照组包括非 H. pylori 感染的胃溃疡和/或胃炎患者（n=50）。患者的采样和分类基于病理发现。进行实时聚合酶链反应以评估 IL-35 和 IL-37 的表达水平。H. pylori 感染性胃炎患者的 IL-35 和 IL-37 表达水平低于未感染组。H. pylori 感染和未感染的胃溃疡和/或胃炎患者的 IL-35 和 IL-37 表达水平存在显著差异。胃溃疡或胃炎的 H. pylori 感染患者的 IL-35 和 IL-37 表达水平无显著差异。白细胞介素 37 和 35 在 H. pylori 感染患者中的表达水平较低。在区分有 H. pylori 感染的胃肠道症状患者和无 H. pylori 感染的有类似症状的患者时，提到的这些白细胞介素可以用作诊断标志物。
