Department of Gastroenterology, Chongming Branch, Shanghai Tenth People's Hospital, Tongji University School of Medicine, No. 66 Xiangyangdong Road, Bao Town, Chongming District, Shanghai, 202157, People's Republic of China.
Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, People's Republic of China.
Sci Rep. 2024 Apr 1;14(1):7683. doi: 10.1038/s41598-024-58407-x.
Helicobacter pylori (H. pylori), known for causing gastric inflammation, gastritis and gastric cancer, prompted our study to investigate the differential expression of cytokines in gastric tissues, which is crucial for understanding H. pylori infection and its potential progression to gastric cancer. Focusing on Il-1β, IL-6, IL-8, IL-12, IL-18, and TNF-α, we analysed gene and protein levels to differentiate between H. pylori-infected and non-infected gastritis. We utilised real-time quantitative polymerase chain reaction (RT-qPCR) for gene quantification, immunohistochemical staining, and ELISA for protein measurement. Gastric samples from patients with gastritis were divided into three groups: (1) non-gastritis (N-group) group, (2) gastritis without H. pylori infection (G-group), and (3) gastritis with H. pylori infection (GH-group), each consisting of 8 samples. Our findings revealed a statistically significant variation in cytokine expression. Generally, cytokine levels were higher in gastritis, but in H. pylori-infected gastritis, IL-1β, IL-6, and IL-8 levels were lower compared to H. pylori-independent gastritis, while IL-12, IL-18, and TNF-α levels were higher. This distinct cytokine expression pattern in H. pylori-infected gastritis underscores a unique inflammatory response, providing deeper insights into its pathogenesis.
幽门螺杆菌（H. pylori），以引起胃炎症、胃炎和胃癌而闻名，促使我们研究调查胃组织中细胞因子的差异表达，这对于理解 H. pylori 感染及其潜在进展为胃癌至关重要。我们专注于白细胞介素 1β（IL-1β）、白细胞介素 6（IL-6）、白细胞介素 8（IL-8）、白细胞介素 12（IL-12）、白细胞介素 18（IL-18）和肿瘤坏死因子-α（TNF-α），分析基因和蛋白质水平，以区分 H. pylori 感染和非感染性胃炎。我们使用实时定量聚合酶链反应（RT-qPCR）进行基因定量、免疫组织化学染色和酶联免疫吸附试验（ELISA）进行蛋白质测量。来自胃炎患者的胃组织样本分为三组：（1）非胃炎（N 组）组、（2）无 H. pylori 感染的胃炎（G 组）和（3）有 H. pylori 感染的胃炎（GH 组），每组包含 8 个样本。我们的发现显示细胞因子表达存在统计学上的显著差异。通常，细胞因子水平在胃炎中较高，但在 H. pylori 感染性胃炎中，IL-1β、IL-6 和 IL-8 水平低于 H. pylori 非依赖性胃炎，而 IL-12、IL-18 和 TNF-α水平较高。H. pylori 感染性胃炎中这种独特的细胞因子表达模式突出了其独特的炎症反应，为其发病机制提供了更深入的了解。
