Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de São João del Rei, Divinópolis, MG, Brazil.
Programa de Pós-Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, BR 364, Km 9.5, Porto Velho, RO, Brazil, 76801-059; Fundação Oswaldo Cruz/Fiocruz Rondônia, Porto Velho, Brazil.
Acta Trop. 2022 Aug;232:106486. doi: 10.1016/j.actatropica.2022.106486. Epub 2022 May 4.
Chagas disease is a zoonotic disease caused by the hemoflagellate Trypanosoma cruzi and transmitted primarily by triatomine vectors. Triatomines are hematophagous insects that feed on a variety of vertebrate hosts. The Chagas disease transmission cycle is closely related to the interactions between vectors, parasites, and vertebrate hosts. Knowledge of triatomine food sources is critical to understanding Chagas disease transmission dynamics. The aim of this study was to identify blood meal sources used by triatomines from different environments in the Brazilian Amazon. A total of 25 captures were conducted in four environments. Triatomine specimens were captured on palm trees and were identified by morphological and morphometric characters. Blood meal sources identification was conducted using a traditional PCR followed by Sanger sequencing of mtDNA cytb gene. Sequencing was successful in 167 specimens and a total of 21 blood meal sources were identified: two reptilians, six birds, and 13 mammals. Among these 21 species, three (Tamandua tetradactyla, Didelphis marsupialis and Rattus rattus) are considered reservoir of T. cruzi. Knowledge of the relationship between triatomines and possible reservoirs can help to elucidate the enzootic cycle of T. cruzi in the Amazon region and guide control strategies for Chagas disease transmission in that region.
恰加斯病是一种由血鞭毛原生动物克氏锥虫引起的人兽共患疾病，主要通过锥蝽传播。锥蝽是吸血昆虫，以多种脊椎动物宿主为食。恰加斯病的传播周期与媒介、寄生虫和脊椎动物宿主之间的相互作用密切相关。了解锥蝽的食物来源对于理解恰加斯病的传播动态至关重要。本研究旨在确定巴西亚马逊地区不同环境中的锥蝽所使用的血液来源。在四个环境中总共进行了 25 次捕获。在棕榈树上捕获锥蝽标本，并通过形态学和形态计量学特征进行鉴定。使用传统 PCR 结合 mtDNA cytb 基因的 Sanger 测序进行血液来源鉴定。167 个标本的测序均成功，共鉴定出 21 种血液来源：两种爬行动物、六种鸟类和 13 种哺乳动物。在这 21 种物种中，有三种（三趾树懒、袋狸和褐家鼠）被认为是克氏锥虫的储存宿主。了解锥蝽与可能的宿主之间的关系有助于阐明亚马逊地区克氏锥虫的地方性循环，并指导该地区恰加斯病传播的控制策略。
