College of Environment, Zhejiang University of Technology, Hangzhou, 310032, PR China.
Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, 310012, PR China.
Environ Pollut. 2022 Aug 1;306:119396. doi: 10.1016/j.envpol.2022.119396. Epub 2022 May 4.
Antibiotic resistance genes (ARGs) and virulence factors (VFs) are critical threats to human health. Their abundance in aquatic ecosystems is maintained and enhanced via selection driven by environmental xenobiotics. However, their activity and expression in these environments under xenobiotic stress remains unknown. Here ARG and VF expression profiles were examined in aquatic microcosms under ciprofloxacin, glyphosate and sertraline hydrochloride treatment. Ciprofloxacin increased total expression of ARGs, particularly multidrug resistance genes. Total expression of ARGs and VFs decreased significantly under glyphosate and sertraline treatments. However, in opportunistic human pathogens, these agents increased expression of both ARGs and VFs. Xenobiotic pollutants, such as the compounds we tested here, have the potential to disrupt microbial ecology, promote resistance, and increase risk to human health. This study systematically evaluated the effects of environmental xenobiotics on transcription of ARGs and VFs, both of which have direct relevance to human health. Transcription of such genes has been overlooked in previous studies.
抗生素耐药基因 (ARGs) 和毒力因子 (VFs) 对人类健康构成重大威胁。它们在水生生态系统中的丰度通过环境污染物驱动的选择得以维持和增强。然而，在这些环境中，它们在 Xenobiotic 胁迫下的活性和表达仍不清楚。在这里，在环丙沙星、草甘膦和盐酸舍曲林处理的水生微宇宙中检查了 ARG 和 VF 的表达谱。环丙沙星增加了 ARGs 的总表达，特别是多药耐药基因。草甘膦和盐酸舍曲林处理后，ARGs 和 VFs 的总表达显著下降。然而，在机会性人类病原体中，这些药物增加了 ARGs 和 VFs 的表达。像我们在这里测试的化合物这样的环境污染物有可能破坏微生物生态系统，促进耐药性，并增加对人类健康的风险。本研究系统地评估了环境 Xenobiotic 对 ARGs 和 VFs 转录的影响，这两者都与人类健康直接相关。在以前的研究中，这些基因的转录被忽视了。
