da Silva Josieli Lima, Mendes Lucas William, Rocha Sandra Mara Barbosa, Antunes Jadson Emanuel Lopes, Oliveira Louise Melo de Souza, Melo Vania Maria Maciel, Oliveira Francisca Andrea Silva, Pereira Arthur Prudêncio de Araujo, Costa Gérson do Nascimento, da Silva Veronica Brito, Gomes Regina Lucia Ferreira, de Alcantara Neto Francisco, Lopes Angela Celis de Almeida, Araujo Ademir Sérgio Ferreira
Plant Genetic Resource Group, Agricultural Science Center, Federal University of Piauí, Teresina, PI, Brazil.
Center for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil.
Microb Ecol. 2023 May;85(4):1423-1433. doi: 10.1007/s00248-022-02028-2. Epub 2022 May 8.
Plants modulate the soil microbiota and select a specific microbial community in the rhizosphere. However, plant domestication reduces genetic diversity, changes plant physiology, and could have an impact on the associated microbiome assembly. Here, we used 16S rRNA gene sequencing to assess the microbial community in the bulk soil and rhizosphere of wild, semi-domesticated, and domesticated genotypes of lima bean (Phaseolus lunatus), to investigate the effect of plant domestication on microbial community assembly. In general, rhizosphere communities were more diverse than bulk soil, but no differences were found among genotypes. Our results showed that the microbial community's structure was different from wild and semi-domesticated as compared to domesticated genotypes. The community similarity decreased 57.67% from wild to domesticated genotypes. In general, the most abundant phyla were Actinobacteria (21.9%), Proteobacteria (20.7%), Acidobacteria (14%), and Firmicutes (9.7%). Comparing the different genotypes, the analysis showed that Firmicutes (Bacillus) was abundant in the rhizosphere of the wild genotypes, while Acidobacteria dominated semi-domesticated plants, and Proteobacteria (including rhizobia) was enriched in domesticated P. lunatus rhizosphere. The domestication process also affected the microbial community network, in which the complexity of connections decreased from wild to domesticated genotypes in the rhizosphere. Together, our work showed that the domestication of P. lunatus shaped rhizosphere microbial communities from taxonomic to a functional level, changing the abundance of specific microbial groups and decreasing the complexity of interactions among them.
植物会调节土壤微生物群，并在根际选择特定的微生物群落。然而，植物驯化会降低遗传多样性、改变植物生理，并且可能会对相关微生物群落的组装产生影响。在这里，我们使用16S rRNA基因测序来评估利马豆（菜豆属）野生型、半驯化型和驯化型基因型的根际和根际土壤中的微生物群落，以研究植物驯化对微生物群落组装的影响。总体而言，根际群落比根际土壤更加多样，但在不同基因型之间未发现差异。我们的结果表明，与驯化型基因型相比，野生型和半驯化型的微生物群落结构有所不同。从野生型到驯化型基因型，群落相似度下降了57.67%。一般来说，最丰富的门类是放线菌门（21.9%）、变形菌门（20.7%）、酸杆菌门（14%）和厚壁菌门（9.7%）。比较不同基因型时，分析表明厚壁菌门（芽孢杆菌属）在野生型基因型的根际中含量丰富，而酸杆菌门在半驯化植物中占主导地位，变形菌门（包括根瘤菌）在驯化的利马豆根际中富集。驯化过程也影响了微生物群落网络，其中根际中连接的复杂性从野生型到驯化型基因型逐渐降低。总之，我们的研究表明，利马豆的驯化从分类学水平到功能水平塑造了根际微生物群落，改变了特定微生物类群的丰度，并降低了它们之间相互作用的复杂性。
