College of Bioengineering, Sichuan University of Science and Engineering, Yibin, People's Republic of China.
Lett Appl Microbiol. 2022 Oct;75(4):881-887. doi: 10.1111/lam.13734. Epub 2022 Jun 23.
Lactobacillus paracasei SLP 16 was obtained from liquor cellar mud, and it was analysed by genome sequencing on Illumina Hiseqq platform. Then the biological information of L. paracasei SLP16 was analysed by ExPasy (website), and the toxin safety of the strain SLP 16 was analysed by PSI/PHI in the virulence factor database VFDB. Through the second-generation DNA sequencing platform technology, the whole genome information of L. paracasei SLP16 was obtained, which showed that the genome size of the strain SLP 16 was 2·65 mol l , and the GC content of the strain SLP 16 was 46·9%. And a total of 3131 genes were detected, including 3067 genes encoding protein and 63 genes encoding RNA. Whole genome analysis showed that L. paracasei SLP16 had five coding genes of F F -ATPase, four coding genes of Na /H antiporter and three coding genes of A-ATPase, which were closely related to the acid tolerance of lactic acid bacteria (LAB). Whole genome analysis of L. paracasei SLP16 showed that SLP 16 had only one CFA synthetic coding gene, and no important BSH coding gene; however, it had F F -ATPase, Na /H antiporter and several two-component regulatory systems, and which were related to bile salt tolerance of LAB. Safety evaluation in L. paracasei SLP16 showed that it did not have the virulence factor coding gene related to toxin. Common antibiotic sensitivity tests showed that L. paracasei SLP16 was resistant to compounds such as sulfamethoxazole, ciprofloxacin, gentamicin and lincomycin. In summary, L. paracasei SLP16 had coding genes closely related to acid tolerance and bile salt tolerance, and no coding gene of virulence factors related to toxins, and few kinds of resistant antibiotics. Therefore, whole genome analysis showed that L. paracasei SLP16 was a safe probiotic strain that can be safely applied.
副干酪乳杆菌 SLP16 从酒窖泥中获得，并在 Illumina Hiseqq 平台上进行基因组测序分析。然后通过 ExPasy（网站）分析副干酪乳杆菌 SLP16 的生物信息，并通过毒力因子数据库 VFDB 中的 PSI/PHI 分析该菌株的毒素安全性。通过第二代 DNA 测序平台技术，获得了副干酪乳杆菌 SLP16 的全基因组信息，结果表明该菌株 SLP16 的基因组大小为 2.65mol/L，GC 含量为 46.9%。共检测到 3131 个基因，包括 3067 个编码蛋白的基因和 63 个编码 RNA 的基因。全基因组分析表明，副干酪乳杆菌 SLP16 有五个编码 F F -ATPase 的基因、四个编码 Na+/H+反向转运蛋白的基因和三个编码 A-ATPase 的基因，这些基因与乳酸菌（LAB）的耐酸能力密切相关。副干酪乳杆菌 SLP16 的全基因组分析表明，SLP16 只有一个 CFA 合成编码基因，没有重要的 BSH 编码基因；然而，它有 F F -ATPase、Na+/H+反向转运蛋白和几个双组分调控系统，这些与 LAB 对胆盐的耐受性有关。副干酪乳杆菌 SLP16 的安全性评价表明，它没有与毒素相关的毒力因子编码基因。常见抗生素药敏试验表明，副干酪乳杆菌 SLP16 对磺胺甲恶唑、环丙沙星、庆大霉素和林可霉素等化合物具有耐药性。综上所述，副干酪乳杆菌 SLP16 具有与耐酸和耐胆盐密切相关的编码基因，没有与毒素相关的毒力因子编码基因，以及几种耐药抗生素。因此，全基因组分析表明，副干酪乳杆菌 SLP16 是一种安全的益生菌菌株，可以安全应用。
