• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高度黏附性乳杆菌副干酪亚种 IBB3423 菌株的比较基因组学和功能分析。

Comparative genomics and functional analysis of a highly adhesive dairy Lactobacillus paracasei subsp. paracasei IBB3423 strain.

机构信息

Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland.

Department of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575, Warsaw, Poland.

出版信息

Appl Microbiol Biotechnol. 2019 Sep;103(18):7617-7634. doi: 10.1007/s00253-019-10010-1. Epub 2019 Jul 29.

DOI:10.1007/s00253-019-10010-1
PMID:31359102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6717177/
Abstract

Various Lactobacillus paracasei strains are found in diverse environments, including dairy and plant materials and the intestinal tract of humans and animals, and are also used in the food industry or as probiotics. In this study, we have isolated a new strain L. paracasei subsp. paracasei IBB3423 from samples of raw cow milk collected in a citizen science project. IBB3423 showed some desired probiotic features such as high adhesion capacity and ability to metabolize inulin. Its complete genome sequence comprising the chromosome of 3,183,386 bp and two plasmids of 5986 bp and 51,211 bp was determined. In silico analysis revealed numerous genes encoding proteins involved in carbohydrate metabolism and of extracellular localization likely supporting interaction with host tissues. In vitro tests confirmed the high adhesion capacity of IBB3423 and showed that it even exceeds that of the highly adhesive L. rhamnosus GG. Curing of the larger plasmid indicated that the adhesive properties depend on the plasmid and thus could be determined by its pilus-encoding spaCBA genes.

摘要

各种副干酪乳杆菌菌株存在于不同的环境中，包括乳制品和植物材料以及人和动物的肠道，也被用于食品工业或作为益生菌。在这项研究中，我们从公民科学项目中收集的生牛乳样本中分离到了一株新的副干酪乳杆菌亚种副干酪乳杆菌 IBB3423。IBB3423 表现出一些理想的益生菌特性，如高粘附能力和菊糖代谢能力。其完整的基因组序列包括一个 3183386bp 的染色体和两个 5986bp 和 51211bp 的质粒。计算机分析显示了许多编码参与碳水化合物代谢的蛋白质的基因，这些蛋白质可能具有细胞外定位，支持与宿主组织的相互作用。体外试验证实了 IBB3423 的高粘附能力，甚至超过了高度粘附的鼠李糖乳杆菌 GG。较大质粒的消除表明，粘附特性取决于质粒，因此可以通过其编码菌毛的 spaCBA 基因来确定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/21c7839bca43/253_2019_10010_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/f8678867d2e5/253_2019_10010_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/547f4861b85b/253_2019_10010_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/5c94cbc2929c/253_2019_10010_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/3f14af18fcb7/253_2019_10010_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/51e2985d8f64/253_2019_10010_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/e34279bdf536/253_2019_10010_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/44d848b2598f/253_2019_10010_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/21c7839bca43/253_2019_10010_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/f8678867d2e5/253_2019_10010_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/547f4861b85b/253_2019_10010_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/5c94cbc2929c/253_2019_10010_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/3f14af18fcb7/253_2019_10010_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/51e2985d8f64/253_2019_10010_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/e34279bdf536/253_2019_10010_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/44d848b2598f/253_2019_10010_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c33/6717177/21c7839bca43/253_2019_10010_Fig8_HTML.jpg

相似文献

1
Comparative genomics and functional analysis of a highly adhesive dairy Lactobacillus paracasei subsp. paracasei IBB3423 strain.高度黏附性乳杆菌副干酪亚种 IBB3423 菌株的比较基因组学和功能分析。
Appl Microbiol Biotechnol. 2019 Sep;103(18):7617-7634. doi: 10.1007/s00253-019-10010-1. Epub 2019 Jul 29.
2
Selection of indigenous Lactobacillus paracasei CD4 and Lactobacillus gastricus BTM 7 as probiotic: assessment of traits combined with principal component analysis.选择本土副干酪乳杆菌CD4和胃乳杆菌BTM 7作为益生菌：结合主成分分析对特性进行评估。
J Appl Microbiol. 2017 May;122(5):1310-1320. doi: 10.1111/jam.13426.
3
Comparative genomic and metabolic analysis of three Lactobacillus paracasei cheese isolates reveals considerable genomic differences in strains from the same niche.对 3 株干酪乳杆菌奶酪分离株的比较基因组学和代谢分析揭示了来自同一生态位的菌株在基因组上存在相当大的差异。
BMC Genomics. 2018 Mar 20;19(1):205. doi: 10.1186/s12864-018-4586-0.
4
Genomic and Functional Characterization of the Unusual pLOCK 0919 Plasmid Harboring the spaCBA Pili Cluster in Lactobacillus casei LOCK 0919.干酪乳杆菌LOCK 0919中携带spaCBA菌毛簇的异常pLOCK 0919质粒的基因组和功能特征分析
Genome Biol Evol. 2015 Dec 3;8(1):202-17. doi: 10.1093/gbe/evv247.
5
Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular -Inulinase of Lactobacillus paracasei Strain W20.双歧杆菌菌株间的共营养作用涉及到副干酪乳杆菌 W20 菌株的胞外菊粉酶。
Appl Environ Microbiol. 2018 Oct 17;84(21). doi: 10.1128/AEM.01539-18. Print 2018 Nov 1.
6
Characterization and Identification of Probiotic Features in Lacticaseibacillus Paracasei Using a Comparative Genomic Analysis Approach.采用比较基因组分析方法对副干酪乳杆菌益生菌特性进行的鉴定和描述。
Probiotics Antimicrob Proteins. 2022 Dec;14(6):1211-1224. doi: 10.1007/s12602-022-09999-1. Epub 2022 Oct 6.
7
Whole-genome sequence and comparative genome analysis of Lactobacillus paracasei DTA93, a promising probiotic lactic acid bacterium.副干酪乳杆菌 DTA93 的全基因组序列和比较基因组分析，一种有前途的益生菌乳酸菌。
Arch Microbiol. 2020 Sep;202(7):1997-2003. doi: 10.1007/s00203-020-01883-2. Epub 2020 Apr 29.
8
Complete Genome Sequence and Carbohydrates-Active EnZymes (CAZymes) Analysis of Lactobacillus paracasei DTA72, a Potential Probiotic Strain with Strong Capability to Use Inulin.副干酪乳杆菌 DTA72 的全基因组序列和碳水化合物活性酶（CAZymes）分析，这是一株具有较强菊粉利用能力的潜在益生菌菌株。
Curr Microbiol. 2020 Oct;77(10):2867-2875. doi: 10.1007/s00284-020-02089-x. Epub 2020 Jul 4.
9
Effect of a novel potential probiotic Lactobacillus paracasei Jlus66 isolated from fermented milk on nonalcoholic fatty liver in rats.从发酵乳中分离得到的新型潜在益生菌干酪乳杆菌 Jlus66 对大鼠非酒精性脂肪肝的影响。
Food Funct. 2017 Dec 13;8(12):4539-4546. doi: 10.1039/c7fo01108c.
10
"Physicochemical, immunomodulatory and safety aspects of milks fermented with Lactobacillus paracasei isolated from kefir"."从开菲尔中分离的副干酪乳杆菌发酵乳的理化特性、免疫调节及安全性方面"。
Food Res Int. 2019 Sep;123:48-55. doi: 10.1016/j.foodres.2019.04.041. Epub 2019 Apr 19.

引用本文的文献

1
The Molecular Weight of Enzymatically Modified Pectic Oligosaccharides from Apple Pomace as a Determinant for Biological and Prebiotic Activity.苹果渣酶法改性果胶寡糖的分子量作为生物活性和益生元活性的决定因素
Molecules. 2024 Dec 26;30(1):46. doi: 10.3390/molecules30010046.
2
Influence of Novel Microcapsulates of Bee Products on Gut Microbiota Modulation and Their Prebiotic and Pro-Adhesive Properties.新型蜂产品微胶囊对肠道微生物群调节的影响及其益生元和促黏附特性。
Molecules. 2024 Jun 9;29(12):2751. doi: 10.3390/molecules29122751.
3
Bioinformatics and its role in the study of the evolution and probiotic potential of lactic acid bacteria.

本文引用的文献

1
Comparative genomic and metabolic analysis of three Lactobacillus paracasei cheese isolates reveals considerable genomic differences in strains from the same niche.对 3 株干酪乳杆菌奶酪分离株的比较基因组学和代谢分析揭示了来自同一生态位的菌株在基因组上存在相当大的差异。
BMC Genomics. 2018 Mar 20;19(1):205. doi: 10.1186/s12864-018-4586-0.
2
A large-scale evaluation of algorithms to calculate average nucleotide identity.计算平均核苷酸一致性的算法的大规模评估。
Antonie Van Leeuwenhoek. 2017 Oct;110(10):1281-1286. doi: 10.1007/s10482-017-0844-4. Epub 2017 Feb 15.
3
MALDI-TOF Mass Spectrometry Enables a Comprehensive and Fast Analysis of Dynamics and Qualities of Stress Responses of Lactobacillus paracasei subsp. paracasei F19.
生物信息学及其在乳酸菌进化与益生菌潜力研究中的作用。
Food Sci Biotechnol. 2022 Aug 10;32(4):389-412. doi: 10.1007/s10068-022-01142-8. eCollection 2023 Mar.
4
Adhesion and Anti-Adhesion Abilities of Potentially Probiotic Lactic Acid Bacteria and Biofilm Eradication of Honeybee ( L.) Pathogens.潜在益生菌乳酸菌的黏附与抗黏附能力和蜜蜂（L.）病原体的生物膜清除。
Molecules. 2022 Dec 15;27(24):8945. doi: 10.3390/molecules27248945.
5
Phenotypic and genotypic characterisation of and yeast isolates from a traditional New Zealand Māori potato starter culture.来自新西兰毛利人传统马铃薯发酵剂培养物的酵母分离株的表型和基因型特征分析。
Curr Res Food Sci. 2022 Aug 19;5:1287-1294. doi: 10.1016/j.crfs.2022.08.004. eCollection 2022.
6
Genomic Insight Into SP5, Reveals Genes and Gene Clusters of Probiotic Interest and Biotechnological Potential.对SP5的基因组洞察揭示了具有益生菌研究价值和生物技术潜力的基因及基因簇。
Front Microbiol. 2022 Jun 16;13:922689. doi: 10.3389/fmicb.2022.922689. eCollection 2022.
7
Variability of Genetic Characters Associated with Probiotic Functions in Species.与物种中益生菌功能相关的遗传特征变异性
Microorganisms. 2022 May 13;10(5):1023. doi: 10.3390/microorganisms10051023.
8
The Edible Plant Microbiome represents a diverse genetic reservoir with functional potential in the human host.食用植物微生物组代表了一个具有功能潜力的多样化遗传资源库，在人类宿主中发挥作用。
Sci Rep. 2021 Dec 15;11(1):24017. doi: 10.1038/s41598-021-03334-4.
9
Potential Probiotic OV3-6 and Its Bioactive Peptide as Alternative Bio-Preservation.潜在益生菌OV3-6及其生物活性肽作为替代生物防腐剂
Foods. 2021 Sep 24;10(10):2264. doi: 10.3390/foods10102264.
10
Effects of carbon concentration, oxygen, and controlled pH on the engineering strain Lactiplantibacillus casei E1 in the production of bioethanol from sugarcane molasses.碳浓度、氧气和可控pH值对工程菌株干酪乳杆菌E1利用甘蔗废蜜生产生物乙醇的影响。
AMB Express. 2021 Jun 27;11(1):95. doi: 10.1186/s13568-021-01257-x.
基质辅助激光解吸电离飞行时间质谱法能够全面快速地分析副干酪乳杆菌副干酪亚种F19应激反应的动力学和特性。
PLoS One. 2016 Oct 26;11(10):e0165504. doi: 10.1371/journal.pone.0165504. eCollection 2016.
4
Genomic and Functional Characterization of the Unusual pLOCK 0919 Plasmid Harboring the spaCBA Pili Cluster in Lactobacillus casei LOCK 0919.干酪乳杆菌LOCK 0919中携带spaCBA菌毛簇的异常pLOCK 0919质粒的基因组和功能特征分析
Genome Biol Evol. 2015 Dec 3;8(1):202-17. doi: 10.1093/gbe/evv247.
5
BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences.BlastKOALA 和 GhostKOALA：KEGG 工具用于基因组和宏基因组序列的功能特征分析。
J Mol Biol. 2016 Feb 22;428(4):726-731. doi: 10.1016/j.jmb.2015.11.006. Epub 2015 Nov 14.
6
The Transporter Classification Database (TCDB): recent advances.转运蛋白分类数据库（TCDB）：最新进展
Nucleic Acids Res. 2016 Jan 4;44(D1):D372-9. doi: 10.1093/nar/gkv1103. Epub 2015 Nov 5.
7
Genomic Diversity of Phages Infecting Probiotic Strains of Lactobacillus paracasei.感染副干酪乳杆菌益生菌株的噬菌体的基因组多样性
Appl Environ Microbiol. 2015 Oct 16;82(1):95-105. doi: 10.1128/AEM.02723-15. Print 2016 Jan 1.
8
Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera.通过对213株乳酸菌及其相关属进行比较基因组学研究，拓展乳酸菌的生物技术潜力。
Nat Commun. 2015 Sep 29;6:8322. doi: 10.1038/ncomms9322.
9
Correlation of Lactobacillus rhamnosus Genotypes and Carbohydrate Utilization Signatures Determined by Phenotype Profiling.通过表型分析确定的鼠李糖乳杆菌基因型与碳水化合物利用特征的相关性
Appl Environ Microbiol. 2015 Aug 15;81(16):5458-70. doi: 10.1128/AEM.00851-15. Epub 2015 Jun 5.
10
Insights into glycogen metabolism in Lactobacillus acidophilus: impact on carbohydrate metabolism, stress tolerance and gut retention.嗜酸乳杆菌糖原代谢的研究进展：对碳水化合物代谢、应激耐受性和肠道留存的影响
Microb Cell Fact. 2014 Nov 20;13:94. doi: 10.1186/s12934-014-0094-3.