College of Science, Hebei Agricultural University, Baoding 071001, China.
College of Science, Hebei Agricultural University, Baoding 071001, China; College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
Food Chem. 2022 Sep 30;389:133121. doi: 10.1016/j.foodchem.2022.133121. Epub 2022 Apr 29.
The preparation of tyrosine-functional novel hypercrosslinked polymer (HCP@Tyr) was achieved by knitting 1,3,5-triphenylbenzene with l-tyrosine via simple Friedel-Crafts reaction. The HCP@Tyr displayed large surface area (1220 m g) and high adsorption capacity (30 mg g) for nitroimidazoles (NDZs). With HCP@Tyr as potent sorbent, an efficient extraction method was developed to simultaneously extract five NDZs from honey and chicken muscle samples before high performance liquid chromatography-diode array detection. Under optimized conditions, the proposed method provided a good linearity in the range of 0.75-200.0 ng g for honey and 1.5-100.0 ng g for chicken muscle sample. Low detection limits (S/N = 3) were obtained to be 0.10-0.24 ng g for honey and 0.15-0.30 ng g for chicken muscle. The method recovery was 80.7-120% with relative standard deviations below 9.2%. The HCP@Tyr based method can serve as a reliable and sensitive tool for detection of NDZs in honey and chicken muscle samples.
通过简单的傅克反应，将 1,3,5-三苯基苯与 l-酪氨酸编织在一起，制备了酪氨酸功能化新型超交联聚合物（HCP@Tyr）。HCP@Tyr 具有大的比表面积（1220 m g）和高的硝基咪唑（NDZs）吸附容量（30 mg g）。以 HCP@Tyr 为有效吸附剂，建立了一种高效的提取方法，可在高效液相色谱-二极管阵列检测前同时从蜂蜜和鸡肉样品中提取五种 NDZs。在优化条件下，该方法在蜂蜜中的线性范围为 0.75-200.0 ng g，在鸡肉中的线性范围为 1.5-100.0 ng g。检测限（S/N = 3）低至 0.10-0.24 ng g 蜂蜜和 0.15-0.30 ng g 鸡肉。方法回收率为 80.7-120%，相对标准偏差低于 9.2%。基于 HCP@Tyr 的方法可作为一种可靠、灵敏的检测蜂蜜和鸡肉样品中 NDZs 的工具。
