College of Science, Hebei Agricultural University, Baoding 071001, China.
College of Science, Hebei Agricultural University, Baoding 071001, China; College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
Food Chem. 2022 Dec 1;396:133694. doi: 10.1016/j.foodchem.2022.133694. Epub 2022 Jul 14.
Three hydroxyl-functionalized hypercrosslinked polymers (HCP-POL, HCP-HQ and HCP-PG) were synthesized by Friedel-Crafts reaction. The HCP-HQ displayed the largest surface area and highest adsorption capacity for chlorophenols (CPs). Thus, the HCP-HQ was further modified with magnetism to obtain M-HCP-HQ. An efficient magnetic solid-phase extraction method with M-HCP-HQ as adsorbent was developed for the first time to simultaneously extract four CPs from water and honey samples before analysis by high performance liquid chromatography-diode array detection. Under optimized conditions, the low detection limits (S/N = 3) were obtained to be 0.06-0.10 ng mL for water and 0.80-1.75 ng g for honey. The method recovery was 80.7%-119%, with relative standard deviations below 9.5%. The enrichment factors of the CPs were in the range of 57-220. The extraction mechanism could be attributed to the strong polar interaction, hydrogen bonding and π-π interactions between the M-HCP-HQ and CPs. The M-HCP-HQ based method can be served as a reliable and sensitive tool for detection CPs in water and honey samples.
三种含三个羟基的超交联聚合物（HCP-POL、HCP-HQ 和 HCP-PG）通过傅克反应合成。HCP-HQ 表现出最大的比表面积和对氯酚（CPs）的最高吸附能力。因此，HCP-HQ 进一步用磁性进行修饰，得到 M-HCP-HQ。首次开发了一种高效的磁性固相萃取方法，以 M-HCP-HQ 作为吸附剂，用于在高效液相色谱-二极管阵列检测之前同时从水样和蜂蜜样品中萃取四种 CPs。在优化条件下，水样的低检测限（S/N=3）为 0.06-0.10ng mL，蜂蜜的低检测限为 0.80-1.75ng g。方法回收率为 80.7%-119%，相对标准偏差低于 9.5%。CPs 的富集因子在 57-220 范围内。萃取机制可归因于 M-HCP-HQ 与 CPs 之间的强极性相互作用、氢键和π-π相互作用。基于 M-HCP-HQ 的方法可以作为一种可靠、灵敏的检测水样和蜂蜜中 CPs 的工具。
