Dzau V J, Ellison K E, Brody T, Ingelfinger J, Pratt R E
Endocrinology. 1987 Jun;120(6):2334-8. doi: 10.1210/endo-120-6-2334.
Previous studies have reported the presence of renin mRNAs in several mouse tissues and angiotensinogen mRNAs in various rat tissues. Clarification as to whether renin and angiotensinogen mRNAs are coexpressed in the same tissues of the same animal species is important for understanding the biology of the tissue renin-angiotensin system. We employed mouse renin cDNA and rat angiotensinogen cDNA to compare tissue distributions of renin and angiotensinogen in RNAs of the rat and mouse. Both cDNA probes readily cross-hybridize with the corresponding mRNA of the other species. Our results demonstrate several patterns of distribution. Renin and angiotensinogen mRNAs are readily detected in kidney and adrenals of both species. In brain and heart, angiotensinogen mRNAs are present in concentrations that far exceed renin mRNA levels in these organs in both species. In mouse and rat livers, angiotensinogen, but not renin, mRNA is demonstrated. In rat testis, only renin mRNA can be detected, whereas in mouse testes both renin and angiotensinogen mRNA are present. In CD-1 male mouse submandibular gland, renin mRNA exists in high concentrations, whereas angiotensinogen mRNA is present in low levels. In contrast, neither renin nor angiotensinogen mRNA could be detected in rat salivary gland. In summary, our study demonstrates the widespread codistribution of renin and angiotensinogen mRNAs in many tissues of both species, allowing for the possibility of local angiotensin production. However, tissue and species differences in these gene expressions also exist. Understanding differential tissue expressions of these genes will provide additional important insight into the biology of the renin-angiotensin system.
先前的研究报道了肾素mRNA在几种小鼠组织中的存在以及血管紧张素原mRNA在各种大鼠组织中的存在。明确肾素和血管紧张素原mRNA是否在同一动物物种的相同组织中共表达，对于理解组织肾素-血管紧张素系统的生物学特性很重要。我们使用小鼠肾素cDNA和大鼠血管紧张素原cDNA来比较大鼠和小鼠RNA中肾素和血管紧张素原的组织分布。两种cDNA探针都能与另一物种的相应mRNA轻松交叉杂交。我们的结果显示了几种分布模式。在两种物种的肾脏和肾上腺中都能很容易地检测到肾素和血管紧张素原mRNA。在大脑和心脏中，两种物种的这些器官中血管紧张素原mRNA的浓度远远超过肾素mRNA水平。在小鼠和大鼠肝脏中，显示有血管紧张素原mRNA，但没有肾素mRNA。在大鼠睾丸中，只能检测到肾素mRNA，而在小鼠睾丸中肾素和血管紧张素原mRNA都存在。在CD-1雄性小鼠下颌下腺中，肾素mRNA以高浓度存在，而血管紧张素原mRNA以低水平存在。相比之下，在大鼠唾液腺中既检测不到肾素mRNA也检测不到血管紧张素原mRNA。总之，我们的研究表明肾素和血管紧张素原mRNA在两种物种的许多组织中广泛共分布，这使得局部产生血管紧张素成为可能。然而，这些基因表达在组织和物种上也存在差异。了解这些基因的差异组织表达将为肾素-血管紧张素系统的生物学特性提供更多重要的见解。
