Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China.
Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5;278:121290. doi: 10.1016/j.saa.2022.121290. Epub 2022 Apr 22.
Detecting and imaging intracellular hypochlorous acid (HClO) is of great importance owning to its prominent role in numerous pathological and physiological processes. In this contribution, a novel AIE-based fluorescent chemosensor has been developed by employing a benzothiazole derivative. The synthesized probe displayed remarkable ratiometric fluorescent response to HClO with a large emission shift (139 nm), resulting in naked-eye fluorescence changes from red to blue. Under the optimal conditions, this probe was capable of quantitatively detecting HClO within 10 s, and possessed good sensitivity and high selectivity toward HClO over other biologically relevant species. Moreover, it has been successfully utilized to image the exogenous and endogenous HClO in living cells through dual channels, and conveniently detect hypochlorous acid solution on test strips with better accuracy, demonstrating its potential for monitoring HClO in biological and environment fields.
检测和成像细胞内次氯酸（HClO）非常重要，因为它在许多病理和生理过程中都起着重要作用。在本研究中，我们开发了一种基于 AIE 的新型荧光化学传感器，该传感器采用苯并噻唑衍生物。合成的探针对 HClO 表现出显著的比率荧光响应，发射位移大（139nm），导致肉眼荧光从红色变为蓝色。在最佳条件下，该探针能够在 10s 内定量检测 HClO，并且对 HClO 具有良好的灵敏度和选择性，优于其他生物相关物质。此外，它已成功用于通过双通道对活细胞中外源和内源性 HClO 进行成像，并能够在试纸上方便地检测次氯酸溶液，具有更好的准确性，表明其在生物和环境领域监测 HClO 的潜力。
