Nechushtai R, Peterson C C, Peter G F, Thornber J P
Eur J Biochem. 1987 Apr 15;164(2):345-50. doi: 10.1111/j.1432-1033.1987.tb11064.x.
The photosystem I (PSI) complex of Lemna gibba, isolated by deriphat/polyacrylamide gel electrophoresis of thylakoids solubilized in glycosidic surfactants, has been fractionated into its two chlorophyll-protein complexes: a core component (CCI) and a light-harvesting component (LHCI), using either non-denaturing gel electrophoresis or ion-exchange chromatography/sucrose gradient centrifugation. Both methods yielded an LHCI component that contained only one apoprotein of approximately 20 kDa. All the chlorophyll b and lutein of the PSI complex is associated with this LHCI preparation. The chlorophyll a/b ratio of this chlorophyll-protein is 2.5, and lutein is essentially the only carotenoid present. While the purified LHCI from Lemna cross-reacts with antibodies raised against spinach LHCPIb of Lam et al. [FEBS Lett. 168, 10-14 (1984)], no cross-reactivity occurred between it and the major light-harvesting chlorophyll-a/b-protein of PSII, LHCII beta. This and a comparison of the amino acid and pigment compositions of the apoproteins of the LHCI and LHCII beta chlorophyll-proteins indicate that these are two distinct but similar chlorophyll-proteins.
通过对溶解于糖苷表面活性剂中的类囊体进行去污剂/聚丙烯酰胺凝胶电泳分离得到的浮萍（Lemna gibba）光系统I（PSI）复合物，利用非变性凝胶电泳或离子交换色谱/蔗糖梯度离心法，已被分离成其两种叶绿素-蛋白复合物：核心组分（CCI）和捕光组分（LHCI）。两种方法都得到了一种仅包含一种约20 kDa脱辅基蛋白的LHCI组分。PSI复合物的所有叶绿素b和叶黄素都与这种LHCI制剂相关。这种叶绿素-蛋白的叶绿素a/b比值为2.5，叶黄素基本上是唯一存在的类胡萝卜素。虽然从浮萍中纯化的LHCI与针对Lam等人[《欧洲生物化学学会联合会快报》168, 10 - 14 (1984)]的菠菜LHCPIb产生的抗体发生交叉反应，但它与PSII的主要捕光叶绿素a/b蛋白LHCIIβ之间没有交叉反应。这以及对LHCI和LHCIIβ叶绿素-蛋白脱辅基蛋白的氨基酸和色素组成的比较表明，它们是两种不同但相似的叶绿素-蛋白。
