Anandan S, Vainstein A, Thornber J P
Department of Biology, University of California, Los Angeles 90024-1606.
FEBS Lett. 1989 Oct 9;256(1-2):150-4. doi: 10.1016/0014-5793(89)81737-5.
Photosystem I (PSI) in barley consists of at least 11 polypeptides of which three have apparent sizes of 15-19 kDa. Two of these polypeptides (subunits III and IV) are constituents of the core complex (CCI), the third is a component of the light-harvesting complex (LHCI). After fractionation of PSI into its CCI and LHCI components, each of the polypeptides has been isolated and its N-terminal region sequenced. We conclude that the gene sequence published for subunit IV of spinach [(1988) FEBS Lett. 237, 108-112] is not that of subunit IV but rather that of the 17 kDA LHCIc pigment protein. We confirm that the published sequence for subunit III [(1988) Curr. Genet. 14, 511-518] is indeed that of subunit III; seemingly conflicting identifications, based on apparent sizes on SDS-PAGE, of which polypeptides are subunits III and IV are probably explained by subunit III's electrophoretic migration rate being dependent on the solvent.
大麦中的光系统I（PSI）至少由11种多肽组成，其中三种多肽的表观大小为15 - 19 kDa。这三种多肽中的两种（亚基III和IV）是核心复合物（CCI）的组成成分，第三种是捕光复合物（LHCI）的组成成分。将PSI分离为其CCI和LHCI成分后，每种多肽都已被分离出来并对其N端区域进行了测序。我们得出结论，已发表的菠菜亚基IV的基因序列[（1988年）《欧洲生物化学学会联合会快报》237, 108 - 112]并非亚基IV的序列，而是17 kDa LHCIc色素蛋白的序列。我们证实已发表的亚基III的序列[（1988年）《当代遗传学》14, 511 - 518]确实是亚基III的序列；基于SDS - PAGE上的表观大小，关于哪些多肽是亚基III和IV的看似相互矛盾的鉴定结果，可能是由于亚基III的电泳迁移率取决于溶剂所致。
