State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China; The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Bioresour Technol. 2022 Jul;355:127264. doi: 10.1016/j.biortech.2022.127264. Epub 2022 May 5.
Antibiotic resistance genes (ARGs) are considered to be a new environmental pollutant and the removal of ARGs from swine manure by anaerobic fermentation was a crucial topic. This research discusses effects of initial pH values (3, 5, 7, 11) on intracellular and extracellular ARGs (iARGs and eARGs) as well as mobile genetic elements (MGEs) during anaerobic fermentation of swine manure had been examined. The initial pH during fermentation was found to be acidic (pH 3 and 5) in results, which was conducive to the removal of six eARGs and seven iARGs. Similarly, intracellular and extracellular MGEs were effectively eliminated with an initial pH of 3 and 5. The abundance of MGEs and four ARGs were enriched with an initial pH of 7 and 11. Acidic conditions can greatly deduce the diversity as well as abundance of the microbial community, ensuing removal of MEGs and ARGs. These findings are critical for risk assessment and management of ARGs.
抗生素抗性基因（ARGs）被认为是一种新型的环境污染物，因此通过厌氧发酵去除猪粪中的 ARGs 是一个至关重要的课题。本研究探讨了初始 pH 值（3、5、7、11）对猪粪厌氧发酵过程中细胞内和细胞外 ARGs（iARGs 和 eARGs）以及移动遗传元件（MGEs）的影响。结果表明，发酵过程中的初始 pH 值为酸性（pH 值为 3 和 5），有利于去除六种 eARGs 和七种 iARGs。同样，初始 pH 值为 3 和 5 时，细胞内和细胞外的 MGEs 也能被有效去除。而初始 pH 值为 7 和 11 时，MGEs 和四种 ARGs 的丰度增加。酸性条件会极大地降低微生物群落的多样性和丰度，从而导致 MEGs 和 ARGs 的去除。这些发现对于 ARGs 的风险评估和管理至关重要。
