Yu Peng, Dong Peiyan, Zou Yina, Wang Hui
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
Bioresour Technol. 2023 Apr;373:128706. doi: 10.1016/j.biortech.2023.128706. Epub 2023 Feb 4.
Effects of various initial pH values (i.e., 3, 5, 7, 11) during anaerobic fermentation of swine manure on intracellular and extracellular antibiotic resistance genes (iARGs and eARGs) and ARG-carrying potential microbial hosts were investigated. The abundance of almost all iARGs and eARGs decreased by 0.1-1.7 logs at pH 3 and pH 5. The abundance of only three iARGs and eARGs decreased by 0.1-0.9 logs at pH 7 and pH 11. Under acidic initial fermentation conditions (pH 3 and pH 5), the ARG removal effect was more pronounced. Acidic conditions (pH 3 and pH 5) significantly reduced the diversity and abundance of the microbial community, thereby eliminating many potential ARG hosts and antibiotic-resistant pathogenic bacteria (ARPB). Therefore, the study results contribute to the investigation of the effects of swine manure anaerobic fermentation on the removal and risk of contamination of ARGs and ARPB.
研究了猪粪厌氧发酵过程中不同初始pH值（即3、5、7、11）对细胞内和细胞外抗生素抗性基因（iARGs和eARGs）以及携带ARG的潜在微生物宿主的影响。在pH 3和pH 5时，几乎所有iARGs和eARGs的丰度下降了0.1-1.7个对数。在pH 7和pH 11时，只有三种iARGs和eARGs的丰度下降了0.1-0.9个对数。在酸性初始发酵条件下（pH 3和pH 5），ARG去除效果更明显。酸性条件（pH 3和pH 5）显著降低了微生物群落的多样性和丰度，从而消除了许多潜在的ARG宿主和耐抗生素病原菌（ARPB）。因此，该研究结果有助于调查猪粪厌氧发酵对ARGs和ARPB去除及污染风险的影响。
