Radiation & Photochemistry Division, Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, India.
Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Int J Biol Macromol. 2022 Jun 15;210:403-414. doi: 10.1016/j.ijbiomac.2022.04.134. Epub 2022 May 6.
Curcumin, a principal component of Curcuma longa, has a long history of being used topically for wound healing. However, poor aqueous solubility of curcumin leads to poor topical absorption. Recently, gelatin based gel has been reported to overcome this issue. However, the release of curcumin from gelatin gel in the bioavailable or easily absorbable form is still a challenge. The present study reports the development of a composite gel prepared from gelatin, F127 and lecithin using temperature dependant gelation and loading of curcumin within it. Notably, the composite gel facilitated the release of curcumin entrapped within vesicles of ~400 nm size. Further, the composite gel exhibited increase in the storage modulus or gel strength, stability, pore size and hydrophobicity as compared to only gelatin gel. Finally, wound healing assay in murine model indicated that curcumin delivered through composite gel showed a significantly faster healing as compared to that delivered through organic solvent. This was also validated by histopathological and biochemical analysis showing better epithelization and collagen synthesis in the group dressed with curcumin containing composite gel. In conclusion, composite gel facilitated the release of bioavailable or easily absorbable curcumin which in turn enhanced the wound healing.
姜黄素是姜黄的主要成分之一，长期以来一直被用于局部伤口愈合。然而，姜黄素的水溶性差导致其局部吸收不良。最近，据报道基于明胶的凝胶可以克服这个问题。然而，姜黄素从明胶凝胶中以生物可利用或易于吸收的形式释放仍然是一个挑战。本研究报告了一种由明胶、F127 和卵磷脂制备的复合凝胶的开发，该凝胶利用温度依赖性凝胶化和姜黄素的负载来制备。值得注意的是，复合凝胶促进了包裹在~400nm 大小囊泡中的姜黄素的释放。此外，与仅明胶凝胶相比，复合凝胶表现出储能模量或凝胶强度、稳定性、孔径和疏水性的增加。最后，在小鼠模型中的伤口愈合试验表明，通过复合凝胶递送的姜黄素与通过有机溶剂递送的姜黄素相比，愈合速度明显更快。这也通过组织病理学和生化分析得到了验证，显示用含有姜黄素的复合凝胶处理的组有更好的上皮化和胶原蛋白合成。总之，复合凝胶促进了生物可利用或易于吸收的姜黄素的释放，从而促进了伤口愈合。
