Zhou Tingting, Zhang Lili, Wang Ying, Mu Qian, Yin Jingyu
School of Space and Environment, Beihang University No. 37, XueYuan Road, Haidian District Beijing 100191 PR China
Shanghai Institute of Applied Physics, Chinese Academy of Sciences No. 239, Zhangheng Rd, Pudong District Shanghai P. R. China.
RSC Adv. 2019 Aug 8;9(42):24617-24626. doi: 10.1039/c9ra03513c. eCollection 2019 Aug 2.
Perovskite nanomaterials (PNMs) have been shown to be promising materials for the effective replacement of conventional energy source materials. With the increasing use of PNMs, they will inevitably enter aquatic environments, giving rise to concerns regarding the environmental impact of PNMs. To fill up the gap in information about the environmental effect of PNMs, was exposed to a typical PNM LaCoO for 48 h, to assess temporal patterns in PNM bioaccumulation and distribution. Synchrotron radiation based micro X-ray fluorescence spectroscopy (μ-XRF) was used to investigate the time dependent spatial distribution of LaCoO. Reactive oxygen species (ROS), superoxide dismutase (SOD) and Na/K-adenosine triphosphatase (ATPase) were measured as key biomarkers. The results showed that oxidative stress was observed at both LaCoO concentrations and Na/K-ATPase was inhibited by high levels of LaCoO. The mode of action of LaCoO was mainly dependent on the metal forms. At low LaCoO levels, food ingestion was the main entry pathway into organisms and LaCoO nanoparticle aggregates accumulated in the gut area. At high LaCoO levels, both waterborne and dietary uptake was observed and the gut and thoracic limbs were the main target sites for LaCoO nanoparticle aggregates and dissolved ions, respectively. LaCoO was not found to translocate in daphnids during the 48 h exposure period at either concentration, suggesting that internalization did not occur. These findings help further our understanding of the fate of PNMs in aquatic organisms, as well as the associated biological responses to PNM exposure.
钙钛矿纳米材料（PNMs）已被证明是有效替代传统能源材料的有前景的材料。随着PNMs使用的增加，它们将不可避免地进入水生环境，引发对PNMs环境影响的担忧。为了填补关于PNMs环境影响信息的空白，将[具体对象]暴露于典型的PNM LaCoO中48小时，以评估PNM生物累积和分布的时间模式。基于同步辐射的微X射线荧光光谱（μ-XRF）用于研究LaCoO随时间的空间分布。测量了活性氧（ROS）、超氧化物歧化酶（SOD）和钠钾-三磷酸腺苷酶（ATPase）作为关键生物标志物。结果表明，在两种LaCoO浓度下均观察到氧化应激，高浓度的LaCoO抑制了钠钾-ATP酶。LaCoO的作用模式主要取决于金属形态。在低LaCoO水平下，食物摄入是进入生物体的主要途径，LaCoO纳米颗粒聚集体在肠道区域积累。在高LaCoO水平下，观察到水体和饮食摄取，肠道和胸肢分别是LaCoO纳米颗粒聚集体和溶解离子的主要靶位点。在48小时暴露期内，无论哪种浓度，在水蚤中均未发现LaCoO发生转运，这表明没有发生内化。这些发现有助于进一步了解PNMs在水生生物中的归宿，以及对PNM暴露的相关生物学反应。
