rs3806265 和 rs4612666 基因与 1 型糖尿病谷氨酸脱羧酶抗体滴度相关。
rs3806265 and rs4612666 of the Gene Are Associated With the Titer of Glutamic Acid Decarboxylase Antibody in Type 1 Diabetes.
机构信息
National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology (Central South University), Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.
出版信息
Front Endocrinol (Lausanne). 2022 Apr 21;13:835054. doi: 10.3389/fendo.2022.835054. eCollection 2022.
BACKGROUND AND AIMS
The NLRP3 gene is reportedly associated with several autoimmune diseases. However, in the Chinese Han population, whether NLRP3 polymorphisms are associated with type 1 diabetes (T1D) is unclear. Therefore, this study examined the associations of rs3806265 and rs4612666 of the NLRP3 gene with T1D susceptibility and the clinical characteristics of Chinese Han T1D patients.
METHODS
In total, 510 classic T1D patients and 531 healthy controls from the Chinese Han population were recruited for a case-control study. rs3806265 and rs4612666 of the NLRP3 gene were genotyped by MassARRAY. Logistic regression analysis and the chi-square test were used to compare the distributions of the alleles and genotypes of rs3806265 and rs4612666. The relationships between rs3806265 and rs4612666 and the clinical characteristics of T1D patients were analyzed by Kruskal-Wallis one-way ANOVA. Student's t test was used to analyze normally distributed data. Bonferroni correction was used for multiple comparisons.
RESULTS
- rs3806265 was associated with glutamic acid decarboxylase antibody (GADA) titers (P = 0.02), and patients with the CC genotype had higher GADA titers than patients with the TT genotype. 2) rs4612666 was also associated with GADA titers (P=0.041). Compared with patients with the CC genotype, patients with the TT genotype had higher GADA titers. 3) rs3806265 and rs4612666 of the NLRP3 gene were not significantly associated with T1D susceptibility under different genetic models.
CONCLUSION
rs3806265 and rs4612666 of the NLRP3 gene were significantly associated with GADA titers in Chinese Han T1D patients.
背景与目的
NLRP3 基因据称与多种自身免疫性疾病有关。然而，在中国汉族人群中，NLRP3 多态性是否与 1 型糖尿病（T1D）有关尚不清楚。因此，本研究探讨了 NLRP3 基因的 rs3806265 和 rs4612666 多态性与中国汉族 T1D 易感性及 T1D 患者临床特征的关系。
方法
采用病例对照研究，共纳入 510 例经典 T1D 患者和 531 例健康对照者，采用 MassARRAY 技术检测 NLRP3 基因的 rs3806265 和 rs4612666 多态性。采用 logistic 回归分析和卡方检验比较 rs3806265 和 rs4612666 等位基因和基因型的分布。采用 Kruskal-Wallis 单向方差分析分析 rs3806265 和 rs4612666 与 T1D 患者临床特征的关系。采用 Student's t 检验分析正态分布数据。采用 Bonferroni 校正进行多重比较。
结果
1）rs3806265 与谷氨酸脱羧酶抗体（GADA）滴度相关（P=0.02），CC 基因型患者的 GADA 滴度高于 TT 基因型患者。2）rs4612666 也与 GADA 滴度相关（P=0.041）。与 CC 基因型患者相比，TT 基因型患者的 GADA 滴度更高。3）在不同遗传模型下，NLRP3 基因的 rs3806265 和 rs4612666 与 T1D 易感性无显著相关性。
结论
NLRP3 基因的 rs3806265 和 rs4612666 与中国汉族 T1D 患者的 GADA 滴度显著相关。
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