Nakano Shu-Ichi, Yamashita Hirofumi, Tanabe Kazuya, Sugimoto Naoki
Department of Nanobiochemistry, Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University 7-1-20, Minatojima-minamimachi, Chuo-ku Kobe 650-0047 Japan
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University 7-1-20, Minatojima-minamimachi, Chuo-ku Kobe 650-0047 Japan.
RSC Adv. 2019 Nov 4;9(61):35820-35824. doi: 10.1039/c9ra06797c. eCollection 2019 Oct 31.
Methods to facilitate the catalytic turnover of ribozymes are required for advancing oligonucleotide-based technologies. This study examined tetraalkylammonium ions for their ability to increase the efficiency of catalytic turnover of a native hammerhead ribozyme. Kinetic analysis showed that large tetraalkylammonium ions significantly increased the turnover rate of the ribozyme and was much more effective than poly(ethylene glycol) (PEG) and urea. The magnitude of the rate increase depended on the concentrations of Mg and tetrapentylammonium ions, and the rate was enhanced by more than 180-fold at the optimal concentrations of these salts. The results provide physical insights into interactions of ribozymes with large cationic molecules through electrostatic forces and steric hindrance.
推进基于寡核苷酸的技术需要促进核酶催化周转的方法。本研究考察了四烷基铵离子提高天然锤头状核酶催化周转效率的能力。动力学分析表明，大四烷基铵离子显著提高了核酶的周转速率，且比聚乙二醇（PEG）和尿素更有效。速率增加的幅度取决于镁离子和四戊基铵离子的浓度，在这些盐的最佳浓度下，速率提高了180多倍。这些结果为核酶与大阳离子分子通过静电力和空间位阻的相互作用提供了物理见解。
