Halban P A, Rhodes C J, Shoelson S E
Diabetologia. 1986 Dec;29(12):893-6. doi: 10.1007/BF00870146.
Evaluating islet function in vitro involves studying both insulin biosynthesis and release. For the former, it is necessary to resolve insulin from its precursor, proinsulin. This has been achieved in the past by various procedures, each of which suffers from major drawbacks in terms of resolution and the time involved. We show here that reversed phase high-performance liquid chromatography (HPLC) outperforms previous methods for separating proinsulin from insulin in islet extracts without any prepurification or concentration steps. This HPLC method is rapid (90 min for a complete cycle, including washing the column) and reproducible, while allowing for unambiguous separation and quantification of proinsulin and insulin.
体外评估胰岛功能涉及研究胰岛素的生物合成和释放。对于前者，有必要将胰岛素与其前体胰岛素原分离。过去通过各种方法实现了这一点，但每种方法在分离度和所需时间方面都存在重大缺陷。我们在此表明，反相高效液相色谱法（HPLC）在分离胰岛提取物中的胰岛素原和胰岛素方面优于以往方法，无需任何预纯化或浓缩步骤。这种HPLC方法快速（一个完整循环需90分钟，包括冲洗柱子）且可重复，同时能够明确分离和定量胰岛素原和胰岛素。
