Liu Tianjiao, Wen Li, Huang Shuai, Han Ting-Li, Zhang Lan, Fu Huijia, Li Junnan, Tong Chao, Qi Hongbo, Saffery Richard, Baker Philip N, Kilby Mark D
State Key Laboratory of Maternal and Fetal Medicine of Chongqing Municipality, Chongqing, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
International Collaborative Laboratory of Reproduction and Development, Ministry of Education, Chongqing Medical University, Chongqing, China.
Front Bioeng Biotechnol. 2022 Apr 12;10:786755. doi: 10.3389/fbioe.2022.786755. eCollection 2022.
To investigate metabolomic perturbations caused by twin-twin transfusion syndrome, metabolic changes associated with fetoscopic laser coagulation in both placental tissue and cord plasma, and to investigate differential metabolites pertinent to varying fetal outcomes, including hemodynamic status, birth weight, and cardiac function, of live-born babies. Placental tissue and cord plasma samples from normal term or uncomplicated preterm-born monochorionic twins and those complicated by twin-twin transfusion syndrome treated with or without fetoscopic laser coagulation were analyzed by high-performance liquid chromatography metabolomic profiling. Sixteen comparisons of different co-twin groups were performed. Partial least squares-discriminant analysis, metabolic pathway analysis, biomarker analysis, and Spearman's correlation analysis were conducted based on differential metabolites used to determine potential biomarkers in different comparisons and metabolites that are pertinent to neonatal birth weight and left ventricular ejection fraction. These metabolomic investigations showed that the cord plasma metabolome has a better performance in discriminating fetuses among different hemodynamic groups than placental tissue. The metabolic alteration of twin-twin transfusion syndrome in these two types of samples centers on fatty acid and lipid metabolism. The fetoscopic laser coagulation procedure improves the metabolomic change brought by this syndrome, making the metabolomes of the treated group less distinguishable from those of the control and preterm birth groups. Certain compounds, especially lipids and lipid-like molecules, are noted to be potential biomarkers of this morbid disease and pertinent to neonatal birth weight and ejection fraction. Fetoscopic laser coagulation can ameliorate the metabolomic alteration caused by twin-twin transfusion syndrome in placental tissue and cord plasma, which are involved mainly in fatty acid and lipid-like molecule metabolism. Certain lipids and lipid-like molecules are helpful in differentiating co-twins of different hemodynamic statuses and are significantly correlated with neonatal birth weight or ejection fraction.
研究双胎输血综合征引起的代谢组学扰动、胎盘组织和脐带血浆中与胎儿镜激光凝固相关的代谢变化，并研究与活产婴儿不同胎儿结局（包括血流动力学状态、出生体重和心脏功能）相关的差异代谢物。通过高效液相色谱代谢组学分析，对足月或无并发症早产的单绒毛膜双胎以及患有双胎输血综合征且接受或未接受胎儿镜激光凝固治疗的双胎的胎盘组织和脐带血浆样本进行分析。对不同同卵双胎组进行了16次比较。基于差异代谢物进行偏最小二乘判别分析、代谢途径分析、生物标志物分析和斯皮尔曼相关性分析，以确定不同比较中的潜在生物标志物以及与新生儿出生体重和左心室射血分数相关的代谢物。这些代谢组学研究表明，脐带血浆代谢组在区分不同血流动力学组的胎儿方面比胎盘组织表现更好。这两种类型样本中双胎输血综合征的代谢改变集中在脂肪酸和脂质代谢上。胎儿镜激光凝固手术改善了该综合征带来的代谢组学变化，使治疗组的代谢组与对照组和早产组的代谢组差异更小。某些化合物，尤其是脂质和类脂质分子，被认为是这种疾病的潜在生物标志物，并且与新生儿出生体重和射血分数相关。胎儿镜激光凝固可以改善胎盘组织和脐带血浆中由双胎输血综合征引起的代谢组学改变，这些改变主要涉及脂肪酸和类脂质分子代谢。某些脂质和类脂质分子有助于区分不同血流动力学状态的同卵双胎，并且与新生儿出生体重或射血分数显著相关。
