• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

成骨细胞衍生的外泌体减轻辐射诱导的造血损伤。

Osteoblast Derived Exosomes Alleviate Radiation- Induced Hematopoietic Injury.

作者信息

Xue Jianqi, Du Ruikai, Ling Shukuan, Song Jinping, Yuan Xinxin, Liu Caizhi, Sun Weijia, Li Yuheng, Zhong Guohui, Wang Yinbo, Yuan Guodong, Jin Xiaoyan, Liu Zizhong, Zhao Dingsheng, Li Youyou, Xing Wenjuan, Fan Yuanyuan, Liu Zifan, Pan Junjie, Zhen Zhen, Zhao Yunzhang, Yang Qinna, Li Jianwei, Chang Yan-Zhong, Li Yingxian

机构信息

Laboratory of Molecular Iron Metabolism, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, College of Life Science, Hebei Normal University, Shijiazhuang, China.

State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.

出版信息

Front Bioeng Biotechnol. 2022 Apr 21;10:850303. doi: 10.3389/fbioe.2022.850303. eCollection 2022.

DOI:10.3389/fbioe.2022.850303
PMID:35528209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9070646/
Abstract

As hematopoietic stem cells can differentiate into all hematopoietic lineages, mitigating the damage to hematopoietic stem cells is important for recovery from overdose radiation injury. Cells in bone marrow microenvironment are essential for hematopoietic stem cells maintenance and protection, and many of the paracrine mediators have been discovered in shaping hematopoietic function. Several recent reports support exosomes as effective regulators of hematopoietic stem cells, but the role of osteoblast derived exosomes in hematopoietic stem cells protection is less understood. Here, we investigated that osteoblast derived exosomes could alleviate radiation damage to hematopoietic stem cells. We show that intravenous injection of osteoblast derived exosomes promoted WBC, lymphocyte, monocyte and hematopoietic stem cells recovery after irradiation significantly. By sequencing osteoblast derived exosomes derived miRNAs and verified , we identified miR-21 is involved in hematopoietic stem cells protection via targeting PDCD4. Collectively, our data demonstrate that osteoblast derived exosomes derived miR-21 is a resultful regulator to radio-protection of hematopoietic stem cells and provide a new strategy for reducing radiation induced hematopoietic injury.

摘要

由于造血干细胞可分化为所有造血谱系，减轻对造血干细胞的损伤对于从过量辐射损伤中恢复至关重要。骨髓微环境中的细胞对于造血干细胞的维持和保护至关重要，并且已经发现许多旁分泌介质在塑造造血功能方面发挥作用。最近的几份报告支持外泌体作为造血干细胞的有效调节剂，但成骨细胞衍生的外泌体在造血干细胞保护中的作用尚不清楚。在这里，我们研究了成骨细胞衍生的外泌体是否可以减轻对造血干细胞的辐射损伤。我们发现静脉注射成骨细胞衍生的外泌体可显著促进照射后白细胞、淋巴细胞、单核细胞和造血干细胞的恢复。通过对成骨细胞衍生的外泌体来源的miRNA进行测序并验证，我们确定miR-21通过靶向PDCD4参与造血干细胞的保护。总的来说，我们的数据表明成骨细胞衍生的外泌体来源的miR-21是造血干细胞放射防护的有效调节剂，并为减少辐射诱导的造血损伤提供了新策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/cf5ab6ba801e/fbioe-10-850303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/e9c38798ebd4/fbioe-10-850303-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/9c5c9b3cafe6/fbioe-10-850303-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/df01c04bddba/fbioe-10-850303-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/dc4b04907214/fbioe-10-850303-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/2784149a3208/fbioe-10-850303-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/cf5ab6ba801e/fbioe-10-850303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/e9c38798ebd4/fbioe-10-850303-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/9c5c9b3cafe6/fbioe-10-850303-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/df01c04bddba/fbioe-10-850303-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/dc4b04907214/fbioe-10-850303-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/2784149a3208/fbioe-10-850303-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9070646/cf5ab6ba801e/fbioe-10-850303-g006.jpg

相似文献

1
Osteoblast Derived Exosomes Alleviate Radiation- Induced Hematopoietic Injury.成骨细胞衍生的外泌体减轻辐射诱导的造血损伤。
Front Bioeng Biotechnol. 2022 Apr 21;10:850303. doi: 10.3389/fbioe.2022.850303. eCollection 2022.
2
microRNA-935-modified bone marrow mesenchymal stem cells-derived exosomes enhance osteoblast proliferation and differentiation in osteoporotic rats.微小 RNA-935 修饰的骨髓间充质干细胞来源的外泌体增强骨质疏松症大鼠成骨细胞的增殖和分化。
Life Sci. 2021 May 1;272:119204. doi: 10.1016/j.lfs.2021.119204. Epub 2021 Feb 10.
3
Bone Marrow Mesenchymal Stem Cells-Derived Exosomal MicroRNA-150-3p Promotes Osteoblast Proliferation and Differentiation in Osteoporosis.骨髓间充质干细胞衍生的外泌体微小RNA-150-3p促进骨质疏松症中成骨细胞的增殖和分化。
Hum Gene Ther. 2021 Jul;32(13-14):717-729. doi: 10.1089/hum.2020.005. Epub 2021 Jan 22.
4
Cardiac progenitor cell-derived exosomes prevent cardiomyocytes apoptosis through exosomal miR-21 by targeting PDCD4.心脏祖细胞衍生的外泌体通过靶向PDCD4的外泌体miR-21预防心肌细胞凋亡。
Cell Death Dis. 2016 Jun 23;7(6):e2277. doi: 10.1038/cddis.2016.181.
5
Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII.富含 miR-214 的骨髓间充质干细胞衍生的外泌体通过靶向 CaMKII 调节心脏干细胞的氧化损伤。
Oxid Med Cell Longev. 2018 Aug 7;2018:4971261. doi: 10.1155/2018/4971261. eCollection 2018.
6
Exosomes Derived from MicroRNA-146a-5p-Enriched Bone Marrow Mesenchymal Stem Cells Alleviate Intracerebral Hemorrhage by Inhibiting Neuronal Apoptosis and Microglial M1 Polarization.源自富含微小RNA-146a-5p的骨髓间充质干细胞的外泌体通过抑制神经元凋亡和小胶质细胞M1极化减轻脑出血。
Drug Des Devel Ther. 2020 Aug 5;14:3143-3158. doi: 10.2147/DDDT.S255828. eCollection 2020.
7
Mouse serum protects against total body irradiation-induced hematopoietic system injury by improving the systemic environment after radiation.鼠血清通过改善辐射后全身环境来预防全身照射引起的造血系统损伤。
Free Radic Biol Med. 2019 Feb 1;131:382-392. doi: 10.1016/j.freeradbiomed.2018.12.021. Epub 2018 Dec 19.
8
Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal which targets .人尿源干细胞通过外泌体靶向 保护大鼠肾缺血/再灌注损伤。
Theranostics. 2020 Jul 25;10(21):9561-9578. doi: 10.7150/thno.42153. eCollection 2020.
9
K562 cell-derived exosomes suppress the adhesive function of bone marrow mesenchymal stem cells via delivery of miR-711.K562 细胞来源的外泌体通过递送 miR-711 抑制骨髓间充质干细胞的黏附功能。
Biochem Biophys Res Commun. 2020 Jan 15;521(3):584-589. doi: 10.1016/j.bbrc.2019.10.096. Epub 2019 Oct 31.
10
Lung-derived exosomes in phosgene-induced acute lung injury regulate the functions of mesenchymal stem cells partially via miR-28-5p.肺源细胞外囊泡在光气诱导的急性肺损伤中通过 miR-28-5p 部分调节间充质干细胞的功能。
Biomed Pharmacother. 2020 Jan;121:109603. doi: 10.1016/j.biopha.2019.109603. Epub 2019 Nov 8.

引用本文的文献

1
Remodeling of the bone marrow microenvironment during acute myeloid leukemia progression.急性髓系白血病进展过程中骨髓微环境的重塑
Ann Transl Med. 2024 Aug 1;12(4):63. doi: 10.21037/atm-23-1824. Epub 2024 Jan 15.
2
New Properties of a Well-Known Antioxidant: Pleiotropic Effects of Human Lactoferrin in Mice Exposed to Gamma Irradiation in a Sublethal Dose.一种知名抗氧化剂的新特性：人乳铁蛋白对亚致死剂量γ射线照射小鼠的多效性作用
Antioxidants (Basel). 2022 Sep 18;11(9):1833. doi: 10.3390/antiox11091833.
3
Protection of the hematopoietic system against radiation-induced damage: drugs, mechanisms, and developments.

本文引用的文献

1
How Hematopoietic Stem Cells Respond to Irradiation: Similarities and Differences between Low and High Doses of Ionizing Radiations.造血干细胞对辐射的反应：低剂量和高剂量电离辐射之间的相似性和差异。
Exp Hematol. 2021 Feb;94:11-19. doi: 10.1016/j.exphem.2020.12.001. Epub 2020 Dec 5.
2
Withdrawal: miR-21-mediated radioresistance occurs via promoting repair of DNA double strand breaks.撤稿：miR-21介导的放射抗性通过促进DNA双链断裂的修复而发生。
J Biol Chem. 2020 May 1;295(18):6250. doi: 10.1074/jbc.W120.013725.
3
Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling.
保护造血系统免受辐射损伤：药物、机制与进展。
Arch Pharm Res. 2022 Aug;45(8):558-571. doi: 10.1007/s12272-022-01400-7. Epub 2022 Aug 11.
辐照诱导骨髓间充质干细胞衰老通过旁分泌信号加重成骨分化功能障碍。
Am J Physiol Cell Physiol. 2020 May 1;318(5):C1005-C1017. doi: 10.1152/ajpcell.00520.2019. Epub 2020 Apr 1.
4
MicroRNA-21 maintains hematopoietic stem cell homeostasis through sustaining the NF-κB signaling pathway in mice.MicroRNA-21 通过维持 NF-κB 信号通路在小鼠中维持造血干细胞的稳态。
Haematologica. 2021 Feb 1;106(2):412-423. doi: 10.3324/haematol.2019.236927.
5
MiR-21 suppresses ox-LDL-induced HUVECs apoptosis by targeting PDCD4.微小RNA-21通过靶向程序性细胞死亡蛋白4抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞凋亡。
Int J Clin Exp Pathol. 2017 Sep 1;10(9):10075-10084. eCollection 2017.
6
MiR-21 attenuates apoptosis-triggered by amyloid-β via modulating PDCD4/ PI3K/AKT/GSK-3β pathway in SH-SY5Y cells.miR-21 通过调控 PDCD4/PI3K/AKT/GSK-3β 通路减轻淀粉样β诱导的 SH-SY5Y 细胞凋亡。
Biomed Pharmacother. 2018 May;101:1003-1007. doi: 10.1016/j.biopha.2018.02.043. Epub 2018 Mar 22.
7
Mesenchymal Stromal Cell-Derived Extracellular Vesicles Provide Long-Term Survival After Total Body Irradiation Without Additional Hematopoietic Stem Cell Support.间充质基质细胞衍生的细胞外囊泡在没有额外造血干细胞支持的情况下提供全身照射后的长期存活。
Stem Cells. 2017 Dec;35(12):2379-2389. doi: 10.1002/stem.2716. Epub 2017 Oct 17.
8
miR‑21 attenuates contrast‑induced renal cell apoptosis by targeting PDCD4.miR-21 通过靶向 PDCD4 减轻对比剂诱导的肾小管细胞凋亡。
Mol Med Rep. 2017 Nov;16(5):6757-6763. doi: 10.3892/mmr.2017.7426. Epub 2017 Sep 5.
9
Overexpression of miR-21 in stem cells improves ovarian structure and function in rats with chemotherapy-induced ovarian damage by targeting PDCD4 and PTEN to inhibit granulosa cell apoptosis.干细胞中miR-21的过表达通过靶向PDCD4和PTEN抑制颗粒细胞凋亡，改善化疗诱导的卵巢损伤大鼠的卵巢结构和功能。
Stem Cell Res Ther. 2017 Aug 14;8(1):187. doi: 10.1186/s13287-017-0641-z.
10
Mesenchymal stem cells deliver exogenous miR-21 via exosomes to inhibit nucleus pulposus cell apoptosis and reduce intervertebral disc degeneration.间充质干细胞通过外泌体传递外源性 miR-21 抑制髓核细胞凋亡，减少椎间盘退变。
J Cell Mol Med. 2018 Jan;22(1):261-276. doi: 10.1111/jcmm.13316. Epub 2017 Aug 14.