Xu Yi, Sun Xin, Chen Jing, Xu Jinbiao, Wei Junshui
Department of Stomatology, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, China.
Appl Bionics Biomech. 2022 Apr 27;2022:8805305. doi: 10.1155/2022/8805305. eCollection 2022.
Salivary gland pleomorphic adenoma (SPA) is a benign neoplasm that can still recur even after radical surgery. To investigate its underlying pathogenesis, here, we examined the significance of lncRNA ENST00000603829 in the proliferation and invasion of SPA.
SPA tissues ( = 30) and adjacent normal tissues (NC; = 30) were collected from SPA patients treated at our hospital from June 2017 to December 2019. The human normal salivary gland epithelial cell line (HSG) and SPA cells (PA30, PA37, and PA116) were cultured. qRT-PCR was used for detecting the expression of cyclin D1 and lncRNA ENST00000603829 in tissues and cells. lncRNA ENST00000603829/cyclin D1 was knocked down or overexpressed in PA116 cells. The expression of cyclin D1 and lncRNA ENST00000603829 in different cell lines was examined using qRT-PCR. Transwell assays and cell counting kit-8 (CCK-8) were used to assess cellular invasion and proliferation. The testing result regarding the apoptosis rate and cell cycle was obtained via flow cytometry. Western blot provided the measurement of cyclin D1 expression in cells.
We observed an upregulation of lncRNA ENST00000603829 and cyclin D1 expression in SPA tissues and cells. Knockdown of lncRNA ENST00000603829 inhibited cell invasion and proliferation, promoting apoptosis and retaining the cells during the G0/G1 phase. However, such effects of lncRNA ENST00000603829 knockdown were inhibited when cyclin D1 was overexpressed.
lncRNA ENST00000603829 can promote the occurrence and development of SPA through increasing cyclin D1 expression.
涎腺多形性腺瘤（SPA）是一种良性肿瘤，即使在根治性手术后仍可能复发。为了探究其潜在的发病机制，在此我们研究了lncRNA ENST00000603829在SPA增殖和侵袭中的意义。
收集2017年6月至2019年12月在我院接受治疗的SPA患者的SPA组织（n = 30）和相邻正常组织（NC；n = 30）。培养人正常涎腺上皮细胞系（HSG）和SPA细胞（PA30、PA37和PA116）。采用qRT-PCR检测组织和细胞中细胞周期蛋白D1和lncRNA ENST00000603829的表达。在PA116细胞中敲低或过表达lncRNA ENST00000603829/细胞周期蛋白D1。使用qRT-PCR检测不同细胞系中细胞周期蛋白D1和lncRNA ENST00000603829的表达。采用Transwell实验和细胞计数试剂盒-8（CCK-8）评估细胞侵袭和增殖。通过流式细胞术获得凋亡率和细胞周期的检测结果。蛋白质免疫印迹法检测细胞中细胞周期蛋白D1的表达。
我们观察到lncRNA ENST00000603829和细胞周期蛋白D1在SPA组织和细胞中的表达上调。敲低lncRNA ENST00000603829可抑制细胞侵袭和增殖，促进凋亡并使细胞停滞在G0/G1期。然而，当细胞周期蛋白D1过表达时，lncRNA ENST00000603829敲低的这些作用受到抑制。
lncRNA ENST00000603829可通过增加细胞周期蛋白D1的表达促进SPA的发生和发展。
