Shen Qian, Jiang Qi, Cong Zhirong, Zhou Yin, Huang Xiaoxiao, Zhu Li, Xu Xiaohong, Qian Juan
Department of Hematology & Lymphoma, Affiliated Tumor Hospital of Nantong University, Nantong, China.
Department of Laboratory, Affiliated Tumor Hospital of Nantong University, Nantong, China.
Ann Transl Med. 2022 Feb;10(4):172. doi: 10.21037/atm-21-6710.
Multiple myeloma (MM) is a B-lymphocyte-derived malignancy. It ranks as the second most common hematological malignancy, with relatively high morbidity and mortality. However, the molecular mechanisms of MM occurrence and development remain elusive. This study found that long non-coding RNA AL928768.3 () was abnormally expressed in MM samples. However, the effect and molecular mechanism of on the occurrence and development of MM remains unclear.
Bone marrow fluids of MM patients (n=54) and volunteers (n=13) were collected and CD138 cells were isolated. The expression level of in MM cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the correlation between the expression level of and the clinicopathological features of patients was analyzed. Lentiviral vectors targeting knockdown were constructed and transfected into cells. After transfection, the effects of knockdown on MM cell proliferation and the cell cycle were detected by the CCK-8 assay, clone formation assay, and flow cytometry. The effect of knockdown on MM cell cycle-related proteins was detected by Western blot. In addition, tumorigenicity experiments were performed in nude mice to detect the effect of knockdown on MM cell proliferation .
was highly expressed in MM patient samples and cell lines, and was significantly correlated with the disease stage of patients. Knockdown of significantly inhibited the proliferation and colony formation of MM cells and induced cell cycle arrest in G0/G1 phase. Western blot analysis showed that knockdown of significantly inhibited the expression of CDK2 and cyclin D1 and promoted the expression of cyclin suppressor p21. Knockdown of significantly inhibited the proliferation of MM cells in nude mice.
was highly expressed in MM patients. Knockdown of this gene significantly inhibited the proliferative ability of MM cells and induced cell cycle arrest in G0/G1 phase. Therefore, may be a novel biological target molecule for the early diagnosis, treatment, and prognostic evaluation of MM patients.
多发性骨髓瘤（MM）是一种B淋巴细胞来源的恶性肿瘤。它是第二常见的血液系统恶性肿瘤，发病率和死亡率相对较高。然而，MM发生发展的分子机制仍不清楚。本研究发现长链非编码RNA AL928768.3（）在MM样本中异常表达。然而，其对MM发生发展的作用及分子机制仍不明确。
收集MM患者（n = 54）和志愿者（n = 13）的骨髓液，分离CD138细胞。通过定量实时聚合酶链反应（qRT-PCR）检测MM细胞中 的表达水平，并分析其表达水平与患者临床病理特征之间的相关性。构建靶向 敲低的慢病毒载体并转染细胞。转染后，通过CCK-8法、克隆形成试验和流式细胞术检测 敲低对MM细胞增殖和细胞周期的影响。通过蛋白质免疫印迹法检测 敲低对MM细胞周期相关蛋白的影响。此外，在裸鼠中进行致瘤性实验，以检测 敲低对MM细胞增殖的影响。
在MM患者样本和细胞系中高表达，且与患者疾病分期显著相关。敲低 可显著抑制MM细胞的增殖和集落形成，并诱导细胞周期停滞于G0/G1期。蛋白质免疫印迹分析显示，敲低 可显著抑制细胞周期蛋白依赖性激酶2（CDK2）和细胞周期蛋白D1的表达，并促进细胞周期蛋白抑制因子p21的表达。敲低 可显著抑制裸鼠中MM细胞的增殖。
在MM患者中高表达。敲低该基因可显著抑制MM细胞的增殖能力，并诱导细胞周期停滞于G0/G1期。因此， 可能是MM患者早期诊断、治疗及预后评估的新型生物靶标分子。
