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癫痫病灶中Th17、调节性T细胞及相关细胞因子的时空变化

The Temporal and Spatial Changes of Th17, Tregs, and Related Cytokines in Epilepsy Lesions.

作者信息

Wei Jingbo, Liu Hui, Liu Ziqi, Jiang Xiaohua, Wang Weiping

机构信息

Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China.

North China University of Science and Technology, Tangshan, Hebei 063210, China.

出版信息

Appl Bionics Biomech. 2022 Apr 26;2022:7871302. doi: 10.1155/2022/7871302. eCollection 2022.

DOI:10.1155/2022/7871302
PMID:35528532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9071937/
Abstract

The cellular and molecular mechanisms in pathogenesis and development of epilepsy are still unclear. Specific inflammatory mediators and immune cells may play an important role. The aim of the present study was to investigate the temporal and spatial changes of Th17, Tregs, and related cytokines in epilepsy lesions. LiCl-pilocarpine-induced temporal lobe epilepsy (TLE) rat models were established, sensorimotor function was examined using modified neurological severity score (mNSS), cognitive function was evaluated by Morris water maze (MWM) test, pathological damages were detected by H&E staining and Nissl staining, helper T cells 17 (Th17), regulatory CD4+ T cells (Tregs), and their related cytokines were detected by Western blotting and immune staining. Results showed that Th17 and its related cytokines in epilepsy lesions played a role mainly at acute phase of epilepsy, and they were positively correlated with the pathological changes in the hippocampus and neurological and cognitive dysfunction caused by epilepsy. Conversely, Tregs and their related cytokines mainly played a role at progressive phase and had the opposite effect. Th17 and Tregs restricted each other during the recovery phase to achieve functional balance. Our results suggested that Th17, Tregs, and related cytokines in epilepsy lesions played an important role in the pathogenesis and development of epilepsy and balancing Th17 and Tregs may be efficacious therapeutics for patients with epilepsy.

摘要

癫痫发病机制和发展过程中的细胞与分子机制仍不清楚。特定的炎症介质和免疫细胞可能起重要作用。本研究的目的是探讨癫痫病灶中辅助性T细胞17（Th17）、调节性T细胞（Tregs）及相关细胞因子的时空变化。建立氯化锂-匹罗卡品诱导的颞叶癫痫（TLE）大鼠模型，采用改良神经功能缺损评分（mNSS）检测感觉运动功能，通过莫里斯水迷宫（MWM）试验评估认知功能，用苏木精-伊红（H&E）染色和尼氏染色检测病理损伤，采用蛋白质印迹法和免疫染色检测辅助性T细胞17（Th17）、调节性CD4+ T细胞（Tregs）及其相关细胞因子。结果显示，癫痫病灶中的Th17及其相关细胞因子主要在癫痫急性期发挥作用，且与海马体的病理变化以及癫痫所致的神经和认知功能障碍呈正相关。相反，Tregs及其相关细胞因子主要在进展期发挥作用，且作用相反。在恢复阶段，Th17和Tregs相互制约以实现功能平衡。我们的结果表明，癫痫病灶中的Th17、Tregs及相关细胞因子在癫痫发病机制和发展过程中起重要作用，平衡Th17和Tregs可能是癫痫患者的有效治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/40cf0762a83d/ABB2022-7871302.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/9444058ebd14/ABB2022-7871302.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/9f8b52834c1f/ABB2022-7871302.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/699941b97ba0/ABB2022-7871302.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/165f624f3d02/ABB2022-7871302.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/0be175158e89/ABB2022-7871302.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/40cf0762a83d/ABB2022-7871302.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/9444058ebd14/ABB2022-7871302.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/9f8b52834c1f/ABB2022-7871302.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/699941b97ba0/ABB2022-7871302.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/165f624f3d02/ABB2022-7871302.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/0be175158e89/ABB2022-7871302.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55bf/9071937/40cf0762a83d/ABB2022-7871302.006.jpg

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