Zheng Man, Ju Weixian, Chen Jijie, Yuan Yan, Zhang Cuifen, Liu Fang, Zhang Fenglei
Dongying People's Hospital Dongying 257091 Shandong China
The People's Hospital of Dongying District Dongying 257100 Shandong China.
RSC Adv. 2019 Aug 9;9(43):24822-24832. doi: 10.1039/c9ra01623f. eCollection 2019 Aug 8.
Scorpion venom represents a significant source of bio-active peptides. However, the anti-inflammatory potency of scorpion venom oligopeptides (CMOs) has not been well explored. In the current study, thirty-five CMOs were isolated, the amino acid sequences were identified, and the anti-inflammatory potency was further explored in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. The results showed that CMO-1 (His-Tyr-Gly-His) demonstrated the best anti-inflammatory potency by attenuating inflammatory cytokine (NO, TNF-α, IL-6, and IL-1β) production. CMO-1 also inhibited IκBα degradation and p65 nuclear translocation and suppressed NF-κB activation. Moreover, CMO-1 inhibited the phosphorylation of ERK, JNK, and p38 MAPKs. It is worth noting that CMO-1 exhibited anti-inflammatory potency; thus, it is a potential anti-inflammatory agent.
蝎毒是生物活性肽的重要来源。然而，蝎毒寡肽（CMOs）的抗炎效力尚未得到充分研究。在本研究中，分离出35种CMOs，鉴定了其氨基酸序列，并在脂多糖（LPS）诱导的RAW264.7巨噬细胞中进一步探究了其抗炎效力。结果表明，CMO-1（His-Tyr-Gly-His）通过减弱炎性细胞因子（NO、TNF-α、IL-6和IL-1β）的产生表现出最佳的抗炎效力。CMO-1还抑制IκBα降解和p65核转位，并抑制NF-κB激活。此外，CMO-1抑制ERK、JNK和p38丝裂原活化蛋白激酶的磷酸化。值得注意的是，CMO-1表现出抗炎效力，因此它是一种潜在的抗炎剂。
