Liu Xiaoyan, Ding Nan, Wang Jun, Chen Honglan, Chen Xinwei, Wang Zhidong, Peng Xincun
Engineering Research Center of Nuclear Technology Application, Ministry of Education, Engineering Research Center of New Energy Technology and Equipment of Jiangxi Province, East China Institute of Technology 418 Guanglan Avenue Nanchang 330013 China
Institute for Electric Light Sources, School of Information Science and Technology, Engineering Research Center of Advanced Lighting Technology, Academy of Engineering and Technology, Fudan University Shanghai 200433 China.
RSC Adv. 2019 Oct 1;9(53):30917-30924. doi: 10.1039/c9ra05504e. eCollection 2019 Sep 26.
Herein, a novel solid-based up-conversion fluorescence resonance energy transfer (FRET) sensor was developed using rhodamine B hydrazide, which provided a selective fluorescence response and suitable affinity towards Cu ions over other biologically relevant metal ions because the Cu ion could promote the hydrolysis of α-amino acid esters of rhodamine B hydrazide and yield the Cu·α-amino acid chelate. This solid-based detection system is more convenient for the detection of Cu based on color change and emission spectra instead of the complicated and tedious measurements than other up-conversion sensors and up-conversion luminescent nanoparticles used as an excitation source; moreover, the proposed system shows high selectivity, minimum photo-damage to living organisms, and high chemical stability.
在此，使用罗丹明B酰肼开发了一种新型的基于固体的上转换荧光共振能量转移（FRET）传感器，该传感器对铜离子具有选择性荧光响应，且与其他生物相关金属离子相比，对铜离子具有合适的亲和力，因为铜离子可促进罗丹明B酰肼的α-氨基酸酯水解并生成铜·α-氨基酸螯合物。与其他上转换传感器以及用作激发源的上转换发光纳米颗粒相比，这种基于固体的检测系统基于颜色变化和发射光谱检测铜更为方便，无需进行复杂繁琐的测量；此外，该系统具有高选择性、对生物体的光损伤最小以及高化学稳定性。
